Baseline serum cardiac troponin i concentrations in Sprague-Dawley, spontaneous hypertensive, Wistar, Wistar-Kyoto, and fisher rats as determined with an ultrasensitive immunoassay

Eugene Herman, Alan Knapton, Elliot Rosen, Jun Zhang, Joel Estis, Sara J. Agee, Quynh Anh Lu, John A. Todd, Steven E Lipshultz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cardiac troponins have proved to be reliable blood biomarkers for identifying a variety of myocardial alterations in humans and animals. Recently, an ultrasensitive cTnI assay (Erenna IA) has been used to demonstrate increases in baseline cTnI resulting from drug-induced myocardial injury in rats, dogs, and monkeys, as well as to document baseline cTnI ranges in Sprague-Dawley (SD) rats. The present study was initiated to use the Erenna cTnI assay to further document baseline cTnI concentrations in normal control animals from multiple strains, including SD, Spontaneous Hypertensive (SHR), Wistar, Wistar-Kyoto (WKY), and Fisher strains. Baseline cTnI concentrations were quantified in all rats tested, and males had higher mean cTnI concentrations than females of the same strain. SHR males had the highest mean cTnI concentrations and the largest cTnI variability. Interestingly, cTnI concentrations increased in castrated SHR compared with unaltered male SHR, whereas cTnI concentrations decreased in ovariectomized SHR compared with unaltered female SHR. These results show significant differences in cTnI concentrations between strains, sexes, and noncardiac surgical alterations in control animals, and identify these as potential contributing factors to cTnI baseline variability that should be taken into account when using ultrasensitive cTnI as a biomarker to assess preclinical cardiotoxicity.

Original languageEnglish
Pages (from-to)653-663
Number of pages11
JournalToxicologic Pathology
Volume39
Issue number4
DOIs
StatePublished - Jun 1 2011

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Troponin
Inbred WKY Rats
Immunoassay
Rats
Animals
Biomarkers
Serum
Assays
Haplorhini
Sprague Dawley Rats
Dogs
Blood
Wounds and Injuries
Pharmaceutical Preparations

Keywords

  • cardiac troponin
  • cardiotoxicity
  • immunoassay
  • myocardial injury
  • rat
  • single-molecule counting

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Cell Biology
  • Molecular Biology

Cite this

Baseline serum cardiac troponin i concentrations in Sprague-Dawley, spontaneous hypertensive, Wistar, Wistar-Kyoto, and fisher rats as determined with an ultrasensitive immunoassay. / Herman, Eugene; Knapton, Alan; Rosen, Elliot; Zhang, Jun; Estis, Joel; Agee, Sara J.; Lu, Quynh Anh; Todd, John A.; Lipshultz, Steven E.

In: Toxicologic Pathology, Vol. 39, No. 4, 01.06.2011, p. 653-663.

Research output: Contribution to journalArticle

Herman, Eugene ; Knapton, Alan ; Rosen, Elliot ; Zhang, Jun ; Estis, Joel ; Agee, Sara J. ; Lu, Quynh Anh ; Todd, John A. ; Lipshultz, Steven E. / Baseline serum cardiac troponin i concentrations in Sprague-Dawley, spontaneous hypertensive, Wistar, Wistar-Kyoto, and fisher rats as determined with an ultrasensitive immunoassay. In: Toxicologic Pathology. 2011 ; Vol. 39, No. 4. pp. 653-663.
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