Bacteriophage SPP1 Chu is an alkaline exonuclease in the SynExo family of viral two-component recombinases

Trina S. Vellani, Richard Myers

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Many DNA viruses concatemerize their genomes as a prerequisite to packaging into capsids. Concatemerization arises from either replication or homologous recombination. Replication is already the target of many antiviral drugs, and viral recombinases are an attractive target for drug design, particularly for combination therapy with replication inhibitors, due to their important supporting role in viral growth. To dissect the molecular mechanisms of viral recombination, we and others previously identified a family of viral nucleases that comprise one component of a conserved, two-component viral recombination system. The nuclease component is related to the exonuclease of phage λ and is common to viruses with linear double-stranded DNA genomes. To test the idea that these viruses have a common strategy for recombination and genome concatemerization, we isolated the previously uncharacterized 34.1 gene from Bacillus subtilis phage SPP1, expressed it in Escherichia coli, purified the protein, and determined its enzymatic properties. Like λ exonuclease, Chu (the product of 34.1) forms an oligomer, is a processive alkaline exonuclease that digests linear double-stranded DNA in a Mg2+-dependent reaction, and shows a preference for 5′-phosphorylated DNA ends. A model for viral recombination, based on the phage λ Red recombination system, is proposed.

Original languageEnglish (US)
Pages (from-to)2465-2474
Number of pages10
JournalJournal of Bacteriology
Volume185
Issue number8
DOIs
StatePublished - Apr 1 2003

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Viral Structures
Exonucleases
Recombinases
Bacteriophages
Genetic Recombination
Genome
DNA
Bacillus Phages
Viruses
DNA Viruses
Capsid
Drug Design
Escherichia coli Proteins
Homologous Recombination
Product Packaging
Bacillus subtilis
Antiviral Agents
Growth
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Bacteriophage SPP1 Chu is an alkaline exonuclease in the SynExo family of viral two-component recombinases. / Vellani, Trina S.; Myers, Richard.

In: Journal of Bacteriology, Vol. 185, No. 8, 01.04.2003, p. 2465-2474.

Research output: Contribution to journalArticle

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