Bacterial population dynamics in three anopheline species: The impact on Plasmodium sporogonic development

Charles B. Pumpuni, James Demaio, Melissa Kent, Jonathan R. Davis, John C. Beier

Research output: Contribution to journalArticle

186 Scopus citations

Abstract

The functional role of bacteria in the midgut of adult mosquitoes is unknown. In this study, we examined the population dynamics of midgut bacteria of laboratory reared Anopheles stephensi, An. gambiae, and An. albimanus. Mosquito midguts were dissected under sterile conditions and examined for the presence of bacteria using standard microbiologic techniques. Ninety percent and 73% (n = 30) of newly emerged An. gambiae and An. stephensi, respectively, harbored bacteria. In contrast, only 17% (n = 23) of An, albimanus harbored any bacteria. The bacterial population increased 11-40-fold in the presence of a blood meal, but then decreased to pre-blood meal levels in 3-5 days. Pseudomonas cepacia. Enterobacter agglomerans, and Flavobacterium spp. were found in all three anopheline species. Midgut bacteria were acquired both transtadially and through the sugar meal. Transtadial transmission was demonstrated by successfully passaging Escherichia coli HS5 from the larval to the adult stage. However, midgut bacteria were acquired more efficiently through the sugar meal than through transtadial passage. An increase in midgut bacterial counts after mosquitoes were exposed to a bacteria/sugar suspension significantly reduced oocyst infection rates and densities in Plasmodium falciparum-infected mosquito cohorts. Since bacteria occur naturally in wild mosquitoes, it may be possible to modify anopheline vector competence using introduced or indigenous bacteria.

Original languageEnglish (US)
Pages (from-to)214-218
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume54
Issue number2
DOIs
StatePublished - Feb 1996

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Bacterial population dynamics in three anopheline species: The impact on Plasmodium sporogonic development'. Together they form a unique fingerprint.

  • Cite this