Bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea

R. J. Phipps, P. J. Torrealba, I. T. Lauredo, S. M. Denas, M. W. Sielczak, A. Ahmed, W. M. Abraham, A. Wanner

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


In vivo instillation of Pasteurella haemolytica (≥ 107 colony-forming units/kg) into a lobar bronchus of sheep produced bacterial pneumonia by 7 days postinoculation. Infection was verified bacteriologically and histologically. Macromolecule secretion and ion and water fluxes were subsequently measured in tracheal tissues in vitro and were compared with values from sham-infected sheep. Macromolecules were radiolabeled with 35SO4 and [3H]threonine, and we measured the secretion of macromolecule-bound radiolabel onto the mucosa. Unidirectional fluxes of Cl-, Na+, and water were measured with radioactive tracers under open-circuit and short-ciruit conditions. Lung infection increased basal secretion of bound 35SO4 (by 189%) and bound [3H]threonine (by 110%). It significantly increased net Na+ absorption under open- and short-circuit conditions and induced open-circuit net absorption of Cl- and water (16 ± 29 μl · cm-2 · h-1). These changes were associated with specific recruitment of neutrophils and elevated levels of arachidonate metabolites (thromboxane B2 and leukotriene B4) in the airways. Thus the bacterial pneumonia-induced changes in tracheal mucus secretion may be the result of airway inflammation.

Original languageEnglish (US)
Pages (from-to)2388-2397
Number of pages10
JournalJournal of applied physiology
Issue number6
StatePublished - 1987

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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