Bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea

R. J. Phipps, P. J. Torrealba, I. T. Lauredo, S. M. Denas, M. W. Sielczak, A. Ahmed, W. M. Abraham, Adam Wanner

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In vivo instillation of Pasteurella haemolytica (≥ 107 colony-forming units/kg) into a lobar bronchus of sheep produced bacterial pneumonia by 7 days postinoculation. Infection was verified bacteriologically and histologically. Macromolecule secretion and ion and water fluxes were subsequently measured in tracheal tissues in vitro and were compared with values from sham-infected sheep. Macromolecules were radiolabeled with 35SO4 and [3H]threonine, and we measured the secretion of macromolecule-bound radiolabel onto the mucosa. Unidirectional fluxes of Cl-, Na+, and water were measured with radioactive tracers under open-circuit and short-ciruit conditions. Lung infection increased basal secretion of bound 35SO4 (by 189%) and bound [3H]threonine (by 110%). It significantly increased net Na+ absorption under open- and short-circuit conditions and induced open-circuit net absorption of Cl- and water (16 ± 29 μl · cm-2 · h-1). These changes were associated with specific recruitment of neutrophils and elevated levels of arachidonate metabolites (thromboxane B2 and leukotriene B4) in the airways. Thus the bacterial pneumonia-induced changes in tracheal mucus secretion may be the result of airway inflammation.

Original languageEnglish
Pages (from-to)2388-2397
Number of pages10
JournalJournal of Applied Physiology
Volume62
Issue number6
StatePublished - Aug 27 1987
Externally publishedYes

Fingerprint

Bacterial Pneumonia
Trachea
Sheep
Threonine
Ions
Water
Mannheimia haemolytica
Radioactive Tracers
Thromboxane B2
Leukotriene B4
Neutrophil Infiltration
Bronchi
Mucus
Infection
Mucous Membrane
Stem Cells
Inflammation
Lung

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Phipps, R. J., Torrealba, P. J., Lauredo, I. T., Denas, S. M., Sielczak, M. W., Ahmed, A., ... Wanner, A. (1987). Bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea. Journal of Applied Physiology, 62(6), 2388-2397.

Bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea. / Phipps, R. J.; Torrealba, P. J.; Lauredo, I. T.; Denas, S. M.; Sielczak, M. W.; Ahmed, A.; Abraham, W. M.; Wanner, Adam.

In: Journal of Applied Physiology, Vol. 62, No. 6, 27.08.1987, p. 2388-2397.

Research output: Contribution to journalArticle

Phipps, RJ, Torrealba, PJ, Lauredo, IT, Denas, SM, Sielczak, MW, Ahmed, A, Abraham, WM & Wanner, A 1987, 'Bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea', Journal of Applied Physiology, vol. 62, no. 6, pp. 2388-2397.
Phipps RJ, Torrealba PJ, Lauredo IT, Denas SM, Sielczak MW, Ahmed A et al. Bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea. Journal of Applied Physiology. 1987 Aug 27;62(6):2388-2397.
Phipps, R. J. ; Torrealba, P. J. ; Lauredo, I. T. ; Denas, S. M. ; Sielczak, M. W. ; Ahmed, A. ; Abraham, W. M. ; Wanner, Adam. / Bacterial pneumonia stimulates macromolecule secretion and ion and water fluxes in sheep trachea. In: Journal of Applied Physiology. 1987 ; Vol. 62, No. 6. pp. 2388-2397.
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