Bacterial community of oolitic carbonate sediments of the Bahamas Archipelago

Mara R. Diaz, Alan M. Piggot, Gregor P. Eberli, James S. Klaus

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The present study characterized bacterial communities associated with oolitic carbonate sediments from the Bahamas Archipelago, ranging from high-energy 'active' to lower energy 'non-active' and 'mat-stabilized' environments. Bacterial communities were analyzed using terminal restriction fragment length polymorphisms (TRFLP), clone analyses of the 16S rRNA gene, confocal laser scanning microscopy (CLSM) and the quantitative phenol-sulfuric acid assay for extracellular polymeric substances (EPS). Confocal imaging of oolitic grains stained with cyanine dye-conjugated lectin and EPS quantification demonstrated that all 3 environments harbored attached biofilm communities, but densities increased from the active to the mat-stabilized environment. Bacterial communities associated with all 3 settings were highly diverse and dominated by Proteobacteria (50 to 61%). Analysis of similarity (ANOSIM) and similarity percentages (SIMPER) revealed significant differences among the 3 environments in the relative abundance of Proteobacteria, Planctomycetes, Cyanobacteria, Chlorobi, and Deinococcus-Thermus. Bacterial primary production in the active shoal environment was associated with Rhodobacteraceae, Ectothiorhodospiraceae, and Chlorobi, whereas the lower energy environments appear to harbor a more complex consortium of aerobic photoautotrophs and anaerobic/aerobic anoxygenic phototrophs. The ubiquitousness of photosynthetizers, along with the presence of aerobic/anaerobic heterotrophic microbes (e.g. denitrifiers, sulfate-reducers, biofilm producers/degraders) and the gradient increase in biofilm production on ooid grains from active to mat-stabilized environments, support the potential involvement of these communities in biomineralization and carbonate precipitation

Original languageEnglish (US)
Pages (from-to)9-24
Number of pages16
JournalMarine Ecology Progress Series
Volume485
DOIs
StatePublished - Jun 27 2013

Keywords

  • 16S rRNA diversity
  • Bacterial community
  • Biofilm
  • Carbonate precipitation
  • Carbonate sediment
  • Ooids

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

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