Abstract
Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the early detection of bacterial infections. Herein, we describe a rapid, selective, and highly sensitive protein based biosensing system employing the Fluorescence Resonance Energy Transfer (FRET) between a protein fusion LuxP-EGFP and 7-diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC). The developed biosensing system, which can detect AI-2 at subnanomolar levels, was successfully applied to detect AI-2 in clinical samples such as saliva and blood serum.
| Original language | English |
|---|---|
| Pages (from-to) | 2608-2614 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 87 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 3 2015 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Bacterial autoinducer-2 detection via an engineered quorum sensing protein. / Raut, Nilesh; Joel, Smita; Pasini, Patrizia; Daunert, Sylvia.
In: Analytical Chemistry, Vol. 87, No. 5, 03.03.2015, p. 2608-2614.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bacterial autoinducer-2 detection via an engineered quorum sensing protein
AU - Raut, Nilesh
AU - Joel, Smita
AU - Pasini, Patrizia
AU - Daunert, Sylvia
PY - 2015/3/3
Y1 - 2015/3/3
N2 - Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the early detection of bacterial infections. Herein, we describe a rapid, selective, and highly sensitive protein based biosensing system employing the Fluorescence Resonance Energy Transfer (FRET) between a protein fusion LuxP-EGFP and 7-diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC). The developed biosensing system, which can detect AI-2 at subnanomolar levels, was successfully applied to detect AI-2 in clinical samples such as saliva and blood serum.
AB - Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the early detection of bacterial infections. Herein, we describe a rapid, selective, and highly sensitive protein based biosensing system employing the Fluorescence Resonance Energy Transfer (FRET) between a protein fusion LuxP-EGFP and 7-diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC). The developed biosensing system, which can detect AI-2 at subnanomolar levels, was successfully applied to detect AI-2 in clinical samples such as saliva and blood serum.
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UR - http://www.scopus.com/inward/citedby.url?scp=84923884586&partnerID=8YFLogxK
U2 - 10.1021/ac504172f
DO - 10.1021/ac504172f
M3 - Article
C2 - 25654248
AN - SCOPUS:84923884586
VL - 87
SP - 2608
EP - 2614
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 5
ER -