Bacterial autoinducer-2 detection via an engineered quorum sensing protein

Nilesh Raut, Smita Joel, Patrizia Pasini, Sylvia Daunert

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the early detection of bacterial infections. Herein, we describe a rapid, selective, and highly sensitive protein based biosensing system employing the Fluorescence Resonance Energy Transfer (FRET) between a protein fusion LuxP-EGFP and 7-diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC). The developed biosensing system, which can detect AI-2 at subnanomolar levels, was successfully applied to detect AI-2 in clinical samples such as saliva and blood serum.

Original languageEnglish
Pages (from-to)2608-2614
Number of pages7
JournalAnalytical Chemistry
Volume87
Issue number5
DOIs
StatePublished - Mar 3 2015

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Proteins
Pathogens
Bacteria
Blood
Fusion reactions
Molecules
N-octanoylhomoserine lactone
Communication
Fluorescence Resonance Energy Transfer
coumarin

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Bacterial autoinducer-2 detection via an engineered quorum sensing protein. / Raut, Nilesh; Joel, Smita; Pasini, Patrizia; Daunert, Sylvia.

In: Analytical Chemistry, Vol. 87, No. 5, 03.03.2015, p. 2608-2614.

Research output: Contribution to journalArticle

Raut, Nilesh ; Joel, Smita ; Pasini, Patrizia ; Daunert, Sylvia. / Bacterial autoinducer-2 detection via an engineered quorum sensing protein. In: Analytical Chemistry. 2015 ; Vol. 87, No. 5. pp. 2608-2614.
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