Abstract
Autoinducer-2 (AI-2) is a Quorum Sensing (QS) molecule utilized by bacteria in interspecies communication. More recently, it is identified to be vital in regulating QS pathways in a number of human and foodborne pathogens. Methods to detect AI-2 in a rapid and highly sensitive manner can help in the early detection of bacterial infections. Herein, we describe a rapid, selective, and highly sensitive protein based biosensing system employing the Fluorescence Resonance Energy Transfer (FRET) between a protein fusion LuxP-EGFP and 7-diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC). The developed biosensing system, which can detect AI-2 at subnanomolar levels, was successfully applied to detect AI-2 in clinical samples such as saliva and blood serum.
Original language | English |
---|---|
Pages (from-to) | 2608-2614 |
Number of pages | 7 |
Journal | Analytical Chemistry |
Volume | 87 |
Issue number | 5 |
DOIs | |
State | Published - Mar 3 2015 |
ASJC Scopus subject areas
- Analytical Chemistry