Abstract
A highly sensitive and selective sensing system for antimonite and arsenite was developed based on genetically engineered bacteria harboring the plasmid pBGD23. In this plasmid, arsR, the gene encoding for the ArsR regulatory protein of the ars operon, is fused to lacZ, the gene encoding for the reporter enzyme β-galactosidase. The expression of β-galactosidase in E. coli strains bearing pBGD23 is controlled by ArsR, and this can be related to the concentration of antimonite/arsenite employed to induce the production of β-galactosidase in the bacteria. ArsR has a high specificity for antimonite/arsenite, thus conferring the developed sensing system with high selectivity. This was demonstrated by evaluating several oxoanions and soft metals as potential interferents. The concentration of β-galactosidase expressed in the bacteria was monitored by chemiluminescence. Using this sensing system, antimonite can be detected at concentrations as low as 10-15M. The importance of the E. coli chromosomal ars operon on the observed response was evaluated by employing a strain of E. coli where the chromosomal ars operon has been deleted. Copyright (C) 1998 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 189-195 |
| Number of pages | 7 |
| Journal | Analytica Chimica Acta |
| Volume | 369 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 24 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Bacteria-based chemiluminescence sensing system using β-galactosidase under the control of the ArsR regulatory protein of the ars operon. / Ramanathan, Sridhar; Shi, Weiping; Rosen, Barry P.; Daunert, Sylvia.
In: Analytica Chimica Acta, Vol. 369, No. 3, 24.08.1998, p. 189-195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bacteria-based chemiluminescence sensing system using β-galactosidase under the control of the ArsR regulatory protein of the ars operon
AU - Ramanathan, Sridhar
AU - Shi, Weiping
AU - Rosen, Barry P.
AU - Daunert, Sylvia
PY - 1998/8/24
Y1 - 1998/8/24
N2 - A highly sensitive and selective sensing system for antimonite and arsenite was developed based on genetically engineered bacteria harboring the plasmid pBGD23. In this plasmid, arsR, the gene encoding for the ArsR regulatory protein of the ars operon, is fused to lacZ, the gene encoding for the reporter enzyme β-galactosidase. The expression of β-galactosidase in E. coli strains bearing pBGD23 is controlled by ArsR, and this can be related to the concentration of antimonite/arsenite employed to induce the production of β-galactosidase in the bacteria. ArsR has a high specificity for antimonite/arsenite, thus conferring the developed sensing system with high selectivity. This was demonstrated by evaluating several oxoanions and soft metals as potential interferents. The concentration of β-galactosidase expressed in the bacteria was monitored by chemiluminescence. Using this sensing system, antimonite can be detected at concentrations as low as 10-15M. The importance of the E. coli chromosomal ars operon on the observed response was evaluated by employing a strain of E. coli where the chromosomal ars operon has been deleted. Copyright (C) 1998 Elsevier Science B.V.
AB - A highly sensitive and selective sensing system for antimonite and arsenite was developed based on genetically engineered bacteria harboring the plasmid pBGD23. In this plasmid, arsR, the gene encoding for the ArsR regulatory protein of the ars operon, is fused to lacZ, the gene encoding for the reporter enzyme β-galactosidase. The expression of β-galactosidase in E. coli strains bearing pBGD23 is controlled by ArsR, and this can be related to the concentration of antimonite/arsenite employed to induce the production of β-galactosidase in the bacteria. ArsR has a high specificity for antimonite/arsenite, thus conferring the developed sensing system with high selectivity. This was demonstrated by evaluating several oxoanions and soft metals as potential interferents. The concentration of β-galactosidase expressed in the bacteria was monitored by chemiluminescence. Using this sensing system, antimonite can be detected at concentrations as low as 10-15M. The importance of the E. coli chromosomal ars operon on the observed response was evaluated by employing a strain of E. coli where the chromosomal ars operon has been deleted. Copyright (C) 1998 Elsevier Science B.V.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032563647&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(98)00244-X
DO - 10.1016/S0003-2670(98)00244-X
M3 - Article
AN - SCOPUS:0032563647
VL - 369
SP - 189
EP - 195
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 3
ER -