B-MYB is essential for normal cell cycle progression and chromosomal stability of embryonic stem cells

Kirill V. Tarasov, Yelena S. Tarasova, Wai Leong Tam, Daniel R. Riordon, Steven T. Elliott, Gabriela Kania, Jinliang Li, Satoshi Yamanaka, David G. Crider, Gianluca Testa, Ronald A. Li, Bing Lim, Colin L. Stewart, Yie Liu, Jennifer E. Van Eyk, Robert P. Wersto, Anna M. Wobus, Kenneth R. Boheler

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Background: The transcription factor B-Myb is present in all proliferating cells, and in mice engineered to remove this gene, embryos die in utero just after implantation due to inner cell mass defects. This lethal phenotype has generally been attributed to a proliferation defect in the cell cycle phase of G1. Methodology/Principal Findings: In the present study, we show that the major cell cycle defect in murine embryonic stem (mES) cells occurs in G2/M. Specifically, knockdown of B-Myb by short-hairpin RNAs results in delayed transit through G2/M, severe mitotic spindle and centrosome defects, and in polyploidy. Moreover, many euploid mES cells that are transiently deficient in B-Myb become aneuploid and can no longer be considered viable. Knockdown of B-Myb in mES cells also decreases Oct4 RNA and protein abundance, while over-expression of B-MYB modestly up-regulates pou5f1 gene expression. The coordinated changes in B-Myb and Oct4 expression are due, at least partly, to the ability of B-Myb to directly modulate pou5f1 gene promoter activity in vitro. Ultimately, the loss of B-Myb and associated loss of Oct4 lead to an increase in early markers of differentiation prior to the activation of caspase-mediated programmed cell death. Conclusions/Significance: Appropriate B-Myb expression is critical to the maintenance of chromosomally stable and pluripotent ES cells, but its absence promotes chromosomal instability that results in either aneuploidy or differentiation-associated cell death.

Original languageEnglish
Article numbere2478
JournalPLoS One
Volume3
Issue number6
DOIs
StatePublished - Jun 25 2008
Externally publishedYes

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Chromosomal Instability
embryonic stem cells
Embryonic Stem Cells
Stem cells
cell cycle
Cell Cycle
Cells
Aneuploidy
Defects
mice
aneuploidy
Cell Death
Cell death
Centrosome
Spindle Apparatus
Polyploidy
Genes
Differentiation Antigens
G1 Phase
Caspases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tarasov, K. V., Tarasova, Y. S., Tam, W. L., Riordon, D. R., Elliott, S. T., Kania, G., ... Boheler, K. R. (2008). B-MYB is essential for normal cell cycle progression and chromosomal stability of embryonic stem cells. PLoS One, 3(6), [e2478]. https://doi.org/10.1371/journal.pone.0002478

B-MYB is essential for normal cell cycle progression and chromosomal stability of embryonic stem cells. / Tarasov, Kirill V.; Tarasova, Yelena S.; Tam, Wai Leong; Riordon, Daniel R.; Elliott, Steven T.; Kania, Gabriela; Li, Jinliang; Yamanaka, Satoshi; Crider, David G.; Testa, Gianluca; Li, Ronald A.; Lim, Bing; Stewart, Colin L.; Liu, Yie; Van Eyk, Jennifer E.; Wersto, Robert P.; Wobus, Anna M.; Boheler, Kenneth R.

In: PLoS One, Vol. 3, No. 6, e2478, 25.06.2008.

Research output: Contribution to journalArticle

Tarasov, KV, Tarasova, YS, Tam, WL, Riordon, DR, Elliott, ST, Kania, G, Li, J, Yamanaka, S, Crider, DG, Testa, G, Li, RA, Lim, B, Stewart, CL, Liu, Y, Van Eyk, JE, Wersto, RP, Wobus, AM & Boheler, KR 2008, 'B-MYB is essential for normal cell cycle progression and chromosomal stability of embryonic stem cells', PLoS One, vol. 3, no. 6, e2478. https://doi.org/10.1371/journal.pone.0002478
Tarasov, Kirill V. ; Tarasova, Yelena S. ; Tam, Wai Leong ; Riordon, Daniel R. ; Elliott, Steven T. ; Kania, Gabriela ; Li, Jinliang ; Yamanaka, Satoshi ; Crider, David G. ; Testa, Gianluca ; Li, Ronald A. ; Lim, Bing ; Stewart, Colin L. ; Liu, Yie ; Van Eyk, Jennifer E. ; Wersto, Robert P. ; Wobus, Anna M. ; Boheler, Kenneth R. / B-MYB is essential for normal cell cycle progression and chromosomal stability of embryonic stem cells. In: PLoS One. 2008 ; Vol. 3, No. 6.
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abstract = "Background: The transcription factor B-Myb is present in all proliferating cells, and in mice engineered to remove this gene, embryos die in utero just after implantation due to inner cell mass defects. This lethal phenotype has generally been attributed to a proliferation defect in the cell cycle phase of G1. Methodology/Principal Findings: In the present study, we show that the major cell cycle defect in murine embryonic stem (mES) cells occurs in G2/M. Specifically, knockdown of B-Myb by short-hairpin RNAs results in delayed transit through G2/M, severe mitotic spindle and centrosome defects, and in polyploidy. Moreover, many euploid mES cells that are transiently deficient in B-Myb become aneuploid and can no longer be considered viable. Knockdown of B-Myb in mES cells also decreases Oct4 RNA and protein abundance, while over-expression of B-MYB modestly up-regulates pou5f1 gene expression. The coordinated changes in B-Myb and Oct4 expression are due, at least partly, to the ability of B-Myb to directly modulate pou5f1 gene promoter activity in vitro. Ultimately, the loss of B-Myb and associated loss of Oct4 lead to an increase in early markers of differentiation prior to the activation of caspase-mediated programmed cell death. Conclusions/Significance: Appropriate B-Myb expression is critical to the maintenance of chromosomally stable and pluripotent ES cells, but its absence promotes chromosomal instability that results in either aneuploidy or differentiation-associated cell death.",
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AU - Elliott, Steven T.

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AU - Li, Ronald A.

AU - Lim, Bing

AU - Stewart, Colin L.

AU - Liu, Yie

AU - Van Eyk, Jennifer E.

AU - Wersto, Robert P.

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AU - Boheler, Kenneth R.

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N2 - Background: The transcription factor B-Myb is present in all proliferating cells, and in mice engineered to remove this gene, embryos die in utero just after implantation due to inner cell mass defects. This lethal phenotype has generally been attributed to a proliferation defect in the cell cycle phase of G1. Methodology/Principal Findings: In the present study, we show that the major cell cycle defect in murine embryonic stem (mES) cells occurs in G2/M. Specifically, knockdown of B-Myb by short-hairpin RNAs results in delayed transit through G2/M, severe mitotic spindle and centrosome defects, and in polyploidy. Moreover, many euploid mES cells that are transiently deficient in B-Myb become aneuploid and can no longer be considered viable. Knockdown of B-Myb in mES cells also decreases Oct4 RNA and protein abundance, while over-expression of B-MYB modestly up-regulates pou5f1 gene expression. The coordinated changes in B-Myb and Oct4 expression are due, at least partly, to the ability of B-Myb to directly modulate pou5f1 gene promoter activity in vitro. Ultimately, the loss of B-Myb and associated loss of Oct4 lead to an increase in early markers of differentiation prior to the activation of caspase-mediated programmed cell death. Conclusions/Significance: Appropriate B-Myb expression is critical to the maintenance of chromosomally stable and pluripotent ES cells, but its absence promotes chromosomal instability that results in either aneuploidy or differentiation-associated cell death.

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