B cells in autoimmune (NZB × NZW)F1 mice show altered IgG isotype switching upon T cell-dependent antigenic stimulation in vitro

Richard L Riley, Mark G. Kruger, Belkis Landa, Jeanne Elia, Andrew Ringel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Humoral immune responses of (NZB × NZW)F1 (BWF1) autoimmune mice to T cell-dependent antigens often exhibit a predominance of IgG2 antibodies, while normal mice produce IgG1 antibodies. In order to determine whether this results from differences in properties of the B cells or the T cells involved, the responses of both primary and secondary BWF1 B cells to the antigen DNP-hemocyanin (Hy) were measured in limiting dilution splenic fragment cultures in the presence of normal T cell help. Furthermore, the capacity of Hy-primed lymph node T cells from BWF1 mice to provide help to BALB c nu nu B cells was determined in modified splenic fragment cultures. These experiments indicated that (a) stimulation of primary BWF1 B cells with DNP-Hy and normal T cell help failed to yield significant numbers of clones which produced any of the IgG isotypes; (b) antigenic stimulation of BWF1 secondary B cell clones also demonstrated a paucity of IgG1, but elevated production of IgG2 isotypes; and (c) Hy-primed BWF1 lymph node T cells were comparable to those derived from BALB c mice in their capacity to provide both help for nu nu B cell responses and modulation of IgG isotype switching. BWF1 B cells apparently differ from normal murine B cells in their capacity to produce IgG antibodies upon T cell-dependent antigenic stimulation.

Original languageEnglish
Pages (from-to)33-45
Number of pages13
JournalClinical Immunology and Immunopathology
Volume58
Issue number1
DOIs
StatePublished - Jan 1 1991

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Immunoglobulin Class Switching
B-Lymphocytes
Immunoglobulin G
T-Lymphocytes
Hemocyanin
Antibodies
Clone Cells
Lymph Nodes
Antigens
In Vitro Techniques
Humoral Immunity

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

B cells in autoimmune (NZB × NZW)F1 mice show altered IgG isotype switching upon T cell-dependent antigenic stimulation in vitro. / Riley, Richard L; Kruger, Mark G.; Landa, Belkis; Elia, Jeanne; Ringel, Andrew.

In: Clinical Immunology and Immunopathology, Vol. 58, No. 1, 01.01.1991, p. 33-45.

Research output: Contribution to journalArticle

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