B cell repertoire diversification precedes immunoglobulin receptor expression

Richard L Riley, D. E. Wylie, N. R. Klinman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

68 monoclonal antibodies specific for the hemagglutinin (HA) of the influenza virus, PR8, were obtained from sIg- bone marrow B cell precursors stimulated in splenic fragment cultures. Reactivity pattern (RP) analysis demonstrated that these anti-HA antibody responses included at least 29 distinguishable clonotypes. Comparison of the specificities of anti-HA antibodies obtained from sIg- bone marrow cells with those obtained from adult spleen cells indicates that the anti-HA repertoires of the two populations are comparable in diversity. Since the sIg- bone marrow B cell precursor pool presumably has not encountered V region-specific regulatory mechanisms in vivo, our data suggest that substantial diversification of the B cell repertoire precedes surface immunoglobulin (sIg) expression and subsequent interaction with environmental regulatory processes.

Original languageEnglish
Pages (from-to)1733-1738
Number of pages6
JournalJournal of Experimental Medicine
Volume158
Issue number5
StatePublished - Dec 1 1983
Externally publishedYes

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B-Cell Antigen Receptors
Hemagglutinins
Immunoglobulins
B-Lymphocytes
Bone Marrow Cells
Anti-Idiotypic Antibodies
Nucleic Acid Regulatory Sequences
Orthomyxoviridae
Antibody Formation
Spleen
Monoclonal Antibodies
Population

ASJC Scopus subject areas

  • Immunology

Cite this

B cell repertoire diversification precedes immunoglobulin receptor expression. / Riley, Richard L; Wylie, D. E.; Klinman, N. R.

In: Journal of Experimental Medicine, Vol. 158, No. 5, 01.12.1983, p. 1733-1738.

Research output: Contribution to journalArticle

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