B cell precursors in senescent mice exhibit decreased recruitment into proliferative compartments and altered expression of Bcl-2 family members

Erin M. Sherwood, Wei Xu, Richard L Riley

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In vitro, aged pro-B cells generally exhibit limited expansion in response to IL-7 when compared to young pro-B cells. CFSE-labeling in vitro indicated that aged mice have a lower frequency of pro-B cells which are capable of undergoing extensive proliferation upon stimulation with exogenous IL-7. Protein levels of the survival molecule Bcl-xL were consistently reduced in IL-7 expanded aged pro-B cells. Levels of both Bcl-2 and Baxα proteins were variable in aged B cell precursors. The expansion of aged pro-B cells in response to IL-7 in vitro correlated inversely with the ratio of the pro-apoptotic protein Baxα to the survival protein Bcl-2. Expansion of aged pro-B cells in vitro is likely dictated by both recruitment of pro-B cells into proliferative compartments and their survival; in aged B cell precursors, the latter is favored by low Baxα to Bcl-2 protein ratios.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalMechanisms of Ageing and Development
Volume124
Issue number2
DOIs
StatePublished - Feb 1 2003

Fingerprint

B-Lymphoid Precursor Cells
Cells
Interleukin-7
bcl-2-Associated X Protein
Apoptosis Regulatory Proteins
Proteins
Labeling
Molecules
In Vitro Techniques

Keywords

  • Apoptosis
  • Pro-B cells
  • Proliferation
  • Senescence

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

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title = "B cell precursors in senescent mice exhibit decreased recruitment into proliferative compartments and altered expression of Bcl-2 family members",
abstract = "In vitro, aged pro-B cells generally exhibit limited expansion in response to IL-7 when compared to young pro-B cells. CFSE-labeling in vitro indicated that aged mice have a lower frequency of pro-B cells which are capable of undergoing extensive proliferation upon stimulation with exogenous IL-7. Protein levels of the survival molecule Bcl-xL were consistently reduced in IL-7 expanded aged pro-B cells. Levels of both Bcl-2 and Baxα proteins were variable in aged B cell precursors. The expansion of aged pro-B cells in response to IL-7 in vitro correlated inversely with the ratio of the pro-apoptotic protein Baxα to the survival protein Bcl-2. Expansion of aged pro-B cells in vitro is likely dictated by both recruitment of pro-B cells into proliferative compartments and their survival; in aged B cell precursors, the latter is favored by low Baxα to Bcl-2 protein ratios.",
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AU - Xu, Wei

AU - Riley, Richard L

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N2 - In vitro, aged pro-B cells generally exhibit limited expansion in response to IL-7 when compared to young pro-B cells. CFSE-labeling in vitro indicated that aged mice have a lower frequency of pro-B cells which are capable of undergoing extensive proliferation upon stimulation with exogenous IL-7. Protein levels of the survival molecule Bcl-xL were consistently reduced in IL-7 expanded aged pro-B cells. Levels of both Bcl-2 and Baxα proteins were variable in aged B cell precursors. The expansion of aged pro-B cells in response to IL-7 in vitro correlated inversely with the ratio of the pro-apoptotic protein Baxα to the survival protein Bcl-2. Expansion of aged pro-B cells in vitro is likely dictated by both recruitment of pro-B cells into proliferative compartments and their survival; in aged B cell precursors, the latter is favored by low Baxα to Bcl-2 protein ratios.

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