Abstract
In vitro, aged pro-B cells generally exhibit limited expansion in response to IL-7 when compared to young pro-B cells. CFSE-labeling in vitro indicated that aged mice have a lower frequency of pro-B cells which are capable of undergoing extensive proliferation upon stimulation with exogenous IL-7. Protein levels of the survival molecule Bcl-xL were consistently reduced in IL-7 expanded aged pro-B cells. Levels of both Bcl-2 and Baxα proteins were variable in aged B cell precursors. The expansion of aged pro-B cells in response to IL-7 in vitro correlated inversely with the ratio of the pro-apoptotic protein Baxα to the survival protein Bcl-2. Expansion of aged pro-B cells in vitro is likely dictated by both recruitment of pro-B cells into proliferative compartments and their survival; in aged B cell precursors, the latter is favored by low Baxα to Bcl-2 protein ratios.
| Original language | English |
|---|---|
| Pages (from-to) | 147-153 |
| Number of pages | 7 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 124 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2003 |
Fingerprint
Keywords
- Apoptosis
- Pro-B cells
- Proliferation
- Senescence
ASJC Scopus subject areas
- Aging
- Biochemistry
- Developmental Biology
- Developmental Neuroscience
Cite this
B cell precursors in senescent mice exhibit decreased recruitment into proliferative compartments and altered expression of Bcl-2 family members. / Sherwood, Erin M.; Xu, Wei; Riley, Richard L.
In: Mechanisms of Ageing and Development, Vol. 124, No. 2, 01.02.2003, p. 147-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - B cell precursors in senescent mice exhibit decreased recruitment into proliferative compartments and altered expression of Bcl-2 family members
AU - Sherwood, Erin M.
AU - Xu, Wei
AU - Riley, Richard L
PY - 2003/2/1
Y1 - 2003/2/1
N2 - In vitro, aged pro-B cells generally exhibit limited expansion in response to IL-7 when compared to young pro-B cells. CFSE-labeling in vitro indicated that aged mice have a lower frequency of pro-B cells which are capable of undergoing extensive proliferation upon stimulation with exogenous IL-7. Protein levels of the survival molecule Bcl-xL were consistently reduced in IL-7 expanded aged pro-B cells. Levels of both Bcl-2 and Baxα proteins were variable in aged B cell precursors. The expansion of aged pro-B cells in response to IL-7 in vitro correlated inversely with the ratio of the pro-apoptotic protein Baxα to the survival protein Bcl-2. Expansion of aged pro-B cells in vitro is likely dictated by both recruitment of pro-B cells into proliferative compartments and their survival; in aged B cell precursors, the latter is favored by low Baxα to Bcl-2 protein ratios.
AB - In vitro, aged pro-B cells generally exhibit limited expansion in response to IL-7 when compared to young pro-B cells. CFSE-labeling in vitro indicated that aged mice have a lower frequency of pro-B cells which are capable of undergoing extensive proliferation upon stimulation with exogenous IL-7. Protein levels of the survival molecule Bcl-xL were consistently reduced in IL-7 expanded aged pro-B cells. Levels of both Bcl-2 and Baxα proteins were variable in aged B cell precursors. The expansion of aged pro-B cells in response to IL-7 in vitro correlated inversely with the ratio of the pro-apoptotic protein Baxα to the survival protein Bcl-2. Expansion of aged pro-B cells in vitro is likely dictated by both recruitment of pro-B cells into proliferative compartments and their survival; in aged B cell precursors, the latter is favored by low Baxα to Bcl-2 protein ratios.
KW - Apoptosis
KW - Pro-B cells
KW - Proliferation
KW - Senescence
UR - http://www.scopus.com/inward/record.url?scp=0037291739&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037291739&partnerID=8YFLogxK
U2 - 10.1016/S0047-6374(02)00042-8
DO - 10.1016/S0047-6374(02)00042-8
M3 - Article
C2 - 12633934
AN - SCOPUS:0037291739
VL - 124
SP - 147
EP - 153
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 2
ER -