B-cell antigen receptor activates transcription factors NFAT (nuclear factor of activated T-cells) and NF-κB (nuclear factor κB) via a mechanism that involves diacylglycerol

P. Antony, J. B. Petro, G. Carlesso, N. P. Shinners, J. Lowe, W. N. Khan

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Engagement of the B-cell antigen receptor (BCR) induces the activation of various transcription factors, including NFAT (nuclear factor of activated T-cells) and NF-κB (nuclear factor κB), which participate in long-term biological responses such as proliferation, survival and differentiation of B-lymphocytes. We addressed the biochemical basis of this process using the DT40 chicken B-cell lymphoma. We discovered that Bruton's tyrosine kinase (BTK) and phospholipase C-γ2 (PLC-γ2) are required to activate NFAT and NF-κB, and to produce the lipid second messenger diacylglycerol in response to BCR cross-linking. Therefore the functional integrity of the BTK/PLC-γ2/diacylglycerol signalling axis is crucial for BCR-directed activation of both transcription factors NFAT and NF-κB.

Original languageEnglish (US)
Pages (from-to)113-115
Number of pages3
JournalBiochemical Society Transactions
Volume32
Issue number1
DOIs
StatePublished - Feb 1 2004
Externally publishedYes

Keywords

  • B lymphocyte
  • Bruton's tyrosine kinase
  • Diacylglycerol
  • Nuclear factor κB (NF-κB)
  • Nuclear factor of activated T-cells (NFAT)
  • Phospholipase C-γ2

ASJC Scopus subject areas

  • Biochemistry

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