Axonal transport reductions in acute experimental allergic encephalomyelitis

Qualitative analysis of the optic nerve

John Guy, E. Ann Ellis, Ernest F. Tark, G. Marion Hope, Narsing A. Rao

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to determine if changes in axonal transport were different in adult animals with acute experimental allergic encephalomyelitis (EAE), in comparison to juvenile animals with chronic EAE, the effects of this acute demyelin-ating disorder on axonal transport were examined in the Optic nerves of adult strain-13 guinea pigs. Utilizing autoradiographic analysis of silver grain counts, both the fast and slow components of orthograde transport were studied at intervals of thirty minutes, three hours, one day and three days after tritiated leucine injection into the vitreous cavity. In order to determine the contribution of fiber loss in acute EAE, optic nerve fiber density was analyzed from electron micrographs of normal and demyelinated nerves. Animals with acute EAE had a decrease in radioactivity at the lamina retinalis and lamina choroidalis after thirty minutes and three hours, and at the lamina scleralis and foci of demyelination after one and three days. A 16% loss of fibers did not account for as much as a 74% reduction in radioactivity with acute EAE. The global reductions in axonal transport observed in acute EAE animals my contribute to their progressive deterioration and eventual demise by lack of delivery of tubule-vesicular mterials for synaptic transmission, axolenmmal proteins for electrogenesis and neurofilamentary components of the cytoskeleton. Moreover, they are unlike the increase of fast axonal transport associated with recovery of physiologic function characteristic of animals with the chronic form of the disease.

Original languageEnglish
Pages (from-to)261-269
Number of pages9
JournalCurrent Eye Research
Volume8
Issue number3
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Axonal Transport
Autoimmune Experimental Encephalomyelitis
Optic Nerve
Radioactivity
Recovery of Function
Demyelinating Diseases
Cytoskeleton
Nerve Fibers
Silver
Leucine
Synaptic Transmission
Guinea Pigs
Chronic Disease
Electrons
Injections
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Axonal transport reductions in acute experimental allergic encephalomyelitis : Qualitative analysis of the optic nerve. / Guy, John; Ellis, E. Ann; Tark, Ernest F.; Hope, G. Marion; Rao, Narsing A.

In: Current Eye Research, Vol. 8, No. 3, 01.01.1989, p. 261-269.

Research output: Contribution to journalArticle

Guy, John ; Ellis, E. Ann ; Tark, Ernest F. ; Hope, G. Marion ; Rao, Narsing A. / Axonal transport reductions in acute experimental allergic encephalomyelitis : Qualitative analysis of the optic nerve. In: Current Eye Research. 1989 ; Vol. 8, No. 3. pp. 261-269.
@article{c9cce74c192a4717a6982f049fa4da91,
title = "Axonal transport reductions in acute experimental allergic encephalomyelitis: Qualitative analysis of the optic nerve",
abstract = "In order to determine if changes in axonal transport were different in adult animals with acute experimental allergic encephalomyelitis (EAE), in comparison to juvenile animals with chronic EAE, the effects of this acute demyelin-ating disorder on axonal transport were examined in the Optic nerves of adult strain-13 guinea pigs. Utilizing autoradiographic analysis of silver grain counts, both the fast and slow components of orthograde transport were studied at intervals of thirty minutes, three hours, one day and three days after tritiated leucine injection into the vitreous cavity. In order to determine the contribution of fiber loss in acute EAE, optic nerve fiber density was analyzed from electron micrographs of normal and demyelinated nerves. Animals with acute EAE had a decrease in radioactivity at the lamina retinalis and lamina choroidalis after thirty minutes and three hours, and at the lamina scleralis and foci of demyelination after one and three days. A 16{\%} loss of fibers did not account for as much as a 74{\%} reduction in radioactivity with acute EAE. The global reductions in axonal transport observed in acute EAE animals my contribute to their progressive deterioration and eventual demise by lack of delivery of tubule-vesicular mterials for synaptic transmission, axolenmmal proteins for electrogenesis and neurofilamentary components of the cytoskeleton. Moreover, they are unlike the increase of fast axonal transport associated with recovery of physiologic function characteristic of animals with the chronic form of the disease.",
author = "John Guy and Ellis, {E. Ann} and Tark, {Ernest F.} and Hope, {G. Marion} and Rao, {Narsing A.}",
year = "1989",
month = "1",
day = "1",
doi = "10.3109/02713688908997568",
language = "English",
volume = "8",
pages = "261--269",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Axonal transport reductions in acute experimental allergic encephalomyelitis

T2 - Qualitative analysis of the optic nerve

AU - Guy, John

AU - Ellis, E. Ann

AU - Tark, Ernest F.

AU - Hope, G. Marion

AU - Rao, Narsing A.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - In order to determine if changes in axonal transport were different in adult animals with acute experimental allergic encephalomyelitis (EAE), in comparison to juvenile animals with chronic EAE, the effects of this acute demyelin-ating disorder on axonal transport were examined in the Optic nerves of adult strain-13 guinea pigs. Utilizing autoradiographic analysis of silver grain counts, both the fast and slow components of orthograde transport were studied at intervals of thirty minutes, three hours, one day and three days after tritiated leucine injection into the vitreous cavity. In order to determine the contribution of fiber loss in acute EAE, optic nerve fiber density was analyzed from electron micrographs of normal and demyelinated nerves. Animals with acute EAE had a decrease in radioactivity at the lamina retinalis and lamina choroidalis after thirty minutes and three hours, and at the lamina scleralis and foci of demyelination after one and three days. A 16% loss of fibers did not account for as much as a 74% reduction in radioactivity with acute EAE. The global reductions in axonal transport observed in acute EAE animals my contribute to their progressive deterioration and eventual demise by lack of delivery of tubule-vesicular mterials for synaptic transmission, axolenmmal proteins for electrogenesis and neurofilamentary components of the cytoskeleton. Moreover, they are unlike the increase of fast axonal transport associated with recovery of physiologic function characteristic of animals with the chronic form of the disease.

AB - In order to determine if changes in axonal transport were different in adult animals with acute experimental allergic encephalomyelitis (EAE), in comparison to juvenile animals with chronic EAE, the effects of this acute demyelin-ating disorder on axonal transport were examined in the Optic nerves of adult strain-13 guinea pigs. Utilizing autoradiographic analysis of silver grain counts, both the fast and slow components of orthograde transport were studied at intervals of thirty minutes, three hours, one day and three days after tritiated leucine injection into the vitreous cavity. In order to determine the contribution of fiber loss in acute EAE, optic nerve fiber density was analyzed from electron micrographs of normal and demyelinated nerves. Animals with acute EAE had a decrease in radioactivity at the lamina retinalis and lamina choroidalis after thirty minutes and three hours, and at the lamina scleralis and foci of demyelination after one and three days. A 16% loss of fibers did not account for as much as a 74% reduction in radioactivity with acute EAE. The global reductions in axonal transport observed in acute EAE animals my contribute to their progressive deterioration and eventual demise by lack of delivery of tubule-vesicular mterials for synaptic transmission, axolenmmal proteins for electrogenesis and neurofilamentary components of the cytoskeleton. Moreover, they are unlike the increase of fast axonal transport associated with recovery of physiologic function characteristic of animals with the chronic form of the disease.

UR - http://www.scopus.com/inward/record.url?scp=0024511631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024511631&partnerID=8YFLogxK

U2 - 10.3109/02713688908997568

DO - 10.3109/02713688908997568

M3 - Article

VL - 8

SP - 261

EP - 269

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 3

ER -