Axonal sprouting and laminin appearance after destruction of glial sheaths

Liria M. Masuda-Nakagawa, Kenneth J Muller, John G. Nicholls

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Laminin, a large extracellular matrix molecule, is associated with axonal outgrowth during development and regeneration of the nervous system in a variety of animals. In the leech central nervous system, laminin immunoreactivity appears after axon injury in advance of the regenerating axons. Although studies of vertebrate nervous system in culture have implicated glial and Schwann cells as possible sources, the cells that deposit laminin at sites crucial for regeneration in the living animal are not known. We have made a direct test to determine whether, in the central nervous system of the leech, cells other than ensheathing glial cells can produce laminin. Ensheathing glial cells of adult leeches were ablated selectively by intracellular injection of a protease. As a result, leech laminin accumulated within 10 days in regions of the central nervous system where it is not normally found, and undamaged, intact axons began to sprout extensively. In normal leeches laminin immunoreactivity is situated only in the base-ment membrane that surrounds the central nervous system, whereas after ablation of ensheathing glia it appeared in spaces through which neurons grew. Within days of ablation of the glial cell, small mobile phagocytes, or microglia, accumulated in the spaces formerly occupied by the glial cell. Microglia were concentrated at precisely the sites of new laminin appearance and axon sprouting. These results suggest that in the animal, as in culture, leech laminin promotes sprouting and that microglia may be responsible for its appearance.

Original languageEnglish
Pages (from-to)4966-4970
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number11
StatePublished - Jun 1 1993
Externally publishedYes

Fingerprint

Laminin
Leeches
Neuroglia
Axons
Microglia
Central Nervous System
Nervous System
Regeneration
Schwann Cells
Phagocytes
Extracellular Matrix
Vertebrates
Peptide Hydrolases
Neurons
Injections
Membranes
Wounds and Injuries

Keywords

  • Desheathment
  • Leech
  • Microglia
  • Regeneration
  • Serotonin

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Axonal sprouting and laminin appearance after destruction of glial sheaths. / Masuda-Nakagawa, Liria M.; Muller, Kenneth J; Nicholls, John G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 11, 01.06.1993, p. 4966-4970.

Research output: Contribution to journalArticle

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