Avian microchromosomes; as shown by prefixation treatment with colchicine, squashing, and haematoxylin staining

Awtar Krishan

Research output: Contribution to journalArticle

Abstract

Small pieces of bird tissue from developing feathers, embryos, embryonic gonads and adult testes are treated before fixation in a 0.25% solution of colchicine diluted with an equal volume of hypotonic Pannett and Compton solution containing KCI, 0.336 gm; CaCl2, 0.160 gm; Na2HP04. 12H2O, 0.172 gm; and NaH2PO4. 4H2O, 0.0172 gm per 1000 ml of double distilled water, for 20-30 min at 37° C. This material is subsequently centrifuged at 1000 rev/min for 1-3 min, fixed with acetic alcohol (1:3) and stained with either Gomori's haematoxylin or by Feulgen's procedure. Preparations are frozen in the freezing chamber of a refrigerator for the separation of the cover slips, then mounted in euparal after dehydration in alcohols. With this technique, unclumped and clear preparations of both macrochromosomes and microchromosomes are obtained regularly.

Original languageEnglish (US)
Pages (from-to)335-337
Number of pages3
JournalBiotechnic and Histochemistry
Volume37
Issue number6
DOIs
StatePublished - Jan 1 1962

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

Fingerprint Dive into the research topics of 'Avian microchromosomes; as shown by prefixation treatment with colchicine, squashing, and haematoxylin staining'. Together they form a unique fingerprint.

  • Cite this