Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis

Philipp Saiko, Maria Ozsvar-Kozma, Geraldine Graser, Andreas Lackner, Michael Grusch, Sibylle Madlener, Georg Krupitza, Walter Jaeger, Mate Hidvegi, Ramp P. Agarwal, Monika Fritzer-Szekeres, Thomas Szekeres

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Avemar (MSC) is a nontoxic fermented wheat germ extract, which has been shown to significantly improve the survival rate in patients suffering from various malignancies. We investigated its effects in sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells. After 48 and 72 h of incubation, Avemar inhibited the growth of sensitive H9 cells with IC50 values of 290 and 200 μg/ml, whereas the growth of 5-FdUrd/Ara-C cross-resistant H9 cells was attenuated with IC50 values of 180 and 145 μg/ml, respectively. Treatment with 300 μg/ml MSC for 48 h caused dosedependent induction of apoptosis in 48% of sensitive H9 cells. In cross-resistant H9 cells, incubation with 200 μg/ml Avemar for 48 h led to 41% of apoptotic tumor cells. Growth arrest of sensitive H9 cells after exposure to various concentrations of MSC occurred mainly in the S phase of the cell cycle, thereby increasing the cell population from 54 to 73% while depleting cells in the G0-G1 phase from 40 to 19%. Growth arrest in cross-resistant H9 cells occurred also mainly in the S phase, increasing the cell population from 45 to 68% while depleting cells in the G0-G1 phase from 45 to 31%. As MSC treatment likely overcomes 5-FdUrd/Ara-C resistance, further investigations to elucidate the exact mechanisms are warranted. We conclude that Avemar exerts a number of beneficial effects which could support conventional chemotherapy of human malignancies.

Original languageEnglish (US)
Pages (from-to)787-791
Number of pages5
JournalOncology Reports
Volume21
Issue number3
DOIs
StatePublished - Apr 23 2009

Keywords

  • Apoptosis
  • Avemar (MSC)
  • Cell cycle
  • Growth inhibition
  • Sensitive and 5-FdUrd/Ara-C crossresistant H9 human lymphoma cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Saiko, P., Ozsvar-Kozma, M., Graser, G., Lackner, A., Grusch, M., Madlener, S., Krupitza, G., Jaeger, W., Hidvegi, M., Agarwal, R. P., Fritzer-Szekeres, M., & Szekeres, T. (2009). Avemar, a nontoxic fermented wheat germ extract, attenuates the growth of sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells through induction of apoptosis. Oncology Reports, 21(3), 787-791. https://doi.org/10.3892/or_00000285