Auxilin facilitates membrane traffic in the early secretory pathway

Jingzhen Ding, Verónica A. Segarra, Shuliang Chen, Huaqing Cai, Sandra Lemmon, Susan Ferro-Novick

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Coat protein complexes contain an inner shell that sorts cargo and an outer shell that helps deform the membrane to give the vesicle its shape. There are three major types of coated vesicles in the cell: COPII, COPI, and clathrin. The COPII coat complex facilitates vesicle budding from the endoplasmic reticulum (ER), while the COPI coat complex performs an analogous function in the Golgi. Clathrin-coated vesicles mediate traffic from the cell surface and between the trans-Golgi and endosome. While the assembly and structure of these coat complexes has been extensively studied, the disassembly of COPII and COPI coats from membranes is less well understood. We describe a proteomic and genetic approach that connects the J-domain chaperone auxilin, which uncoats clathrin-coated vesicles, to COPII and COPI coat complexes. Consistent with a functional role for auxilin in the early secretory pathway, auxilin binds to COPII and COPI coat subunits. Furthermore, ER-Golgi and intra-Golgi traffic is delayed at 15°C in swa2Δ mutant cells, which lack auxilin. In the case of COPII vesicles, we link this delay to a defect in vesicle fusion. We propose that auxilin acts as a chaperone and/or uncoating factor for transport vesicles that act in the early secretory pathway.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalMolecular Biology of the Cell
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2016

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Auxilins
Coat Protein Complex I
Secretory Pathway
Membranes
Clathrin-Coated Vesicles
Endoplasmic Reticulum
Coated Vesicles
Transport Vesicles
Clathrin
Endosomes
Capsid Proteins
Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Auxilin facilitates membrane traffic in the early secretory pathway. / Ding, Jingzhen; Segarra, Verónica A.; Chen, Shuliang; Cai, Huaqing; Lemmon, Sandra; Ferro-Novick, Susan.

In: Molecular Biology of the Cell, Vol. 27, No. 1, 01.01.2016, p. 127-136.

Research output: Contribution to journalArticle

Ding, Jingzhen ; Segarra, Verónica A. ; Chen, Shuliang ; Cai, Huaqing ; Lemmon, Sandra ; Ferro-Novick, Susan. / Auxilin facilitates membrane traffic in the early secretory pathway. In: Molecular Biology of the Cell. 2016 ; Vol. 27, No. 1. pp. 127-136.
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