Abstract
RNase E plays a central role in controlling mRNA degradation in E. coli. We have investigated the mechanism of RNase E autoregulation. Our data indicate that RNase E autoregulates its synthesis by controlling the decay rate of its own transcript (rne mRNA), which is unusually sensitive to the level of cellular RNase E activity. Feedback regulation is mediated in cis by the rne 5' untranslated region (5' UTR), which can confer this property onto heterologous mRNAs to which it is fused. The marked sensitivity of rne mRNA to regulation by RNase E is also due in part to the susceptibility of nascent rne transcripts to RNase E-mediated degradation.
| Original language | English |
|---|---|
| Pages (from-to) | 85-88 |
| Number of pages | 4 |
| Journal | Nucleic acids symposium series |
| Issue number | 33 |
| State | Published - Dec 1 1995 |
| Externally published | Yes |
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Autoregulation of RNase E synthesis in Escherichia coli. / Jain, Chaitanya; Belasco, J. G.
In: Nucleic acids symposium series, No. 33, 01.12.1995, p. 85-88.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Autoregulation of RNase E synthesis in Escherichia coli.
AU - Jain, Chaitanya
AU - Belasco, J. G.
PY - 1995/12/1
Y1 - 1995/12/1
N2 - RNase E plays a central role in controlling mRNA degradation in E. coli. We have investigated the mechanism of RNase E autoregulation. Our data indicate that RNase E autoregulates its synthesis by controlling the decay rate of its own transcript (rne mRNA), which is unusually sensitive to the level of cellular RNase E activity. Feedback regulation is mediated in cis by the rne 5' untranslated region (5' UTR), which can confer this property onto heterologous mRNAs to which it is fused. The marked sensitivity of rne mRNA to regulation by RNase E is also due in part to the susceptibility of nascent rne transcripts to RNase E-mediated degradation.
AB - RNase E plays a central role in controlling mRNA degradation in E. coli. We have investigated the mechanism of RNase E autoregulation. Our data indicate that RNase E autoregulates its synthesis by controlling the decay rate of its own transcript (rne mRNA), which is unusually sensitive to the level of cellular RNase E activity. Feedback regulation is mediated in cis by the rne 5' untranslated region (5' UTR), which can confer this property onto heterologous mRNAs to which it is fused. The marked sensitivity of rne mRNA to regulation by RNase E is also due in part to the susceptibility of nascent rne transcripts to RNase E-mediated degradation.
UR - http://www.scopus.com/inward/record.url?scp=0029438136&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029438136&partnerID=8YFLogxK
M3 - Article
C2 - 8643409
AN - SCOPUS:0029438136
SP - 85
EP - 88
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
SN - 0261-3166
IS - 33
ER -