Autophosphorylation and ADP regulate the Ca2+-dependent interaction of recoverin with rhodopsin kinase

Daulet K. Satpaev, Ching Kang Chen, Anthony Scotti, Melvin I. Simon, James B. Hurley, Vladlen Z Slepak

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Recoverin is a 23 kDa myristoylated Ca2+-binding protein that inhibits rhodopsin kinase. We have used surface plasmon resonance to investigate the influences of Ca2+, myristoylation, and adenine nucleotides on the recoverin-rhodopsin kinase interaction. Our analyses confirmed that Ca2+ is required for recoverin to bind RK. Myristoylation had little effect on the affinity of recoverin for the kinase, but it raised the K0.5 for Ca2+ from 150 nM for nonacylated recoverin to 400 nM for myristoylated recoverin. Finally, our studies also revealed two separate and previously unreported effects of adenine nucleotides on the recoverin-rhodopsin kinase binding. The interaction is weakened by autophosphorylation of the kinase, and it is strengthened by the presence of ADP.

Original languageEnglish
Pages (from-to)10256-10262
Number of pages7
JournalBiochemistry
Volume37
Issue number28
DOIs
StatePublished - Jul 14 1998

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G-Protein-Coupled Receptor Kinase 1
Recoverin
Adenosine Diphosphate
Adenine Nucleotides
Phosphotransferases
Surface Plasmon Resonance
Surface plasmon resonance
Carrier Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Autophosphorylation and ADP regulate the Ca2+-dependent interaction of recoverin with rhodopsin kinase. / Satpaev, Daulet K.; Chen, Ching Kang; Scotti, Anthony; Simon, Melvin I.; Hurley, James B.; Slepak, Vladlen Z.

In: Biochemistry, Vol. 37, No. 28, 14.07.1998, p. 10256-10262.

Research output: Contribution to journalArticle

Satpaev, Daulet K. ; Chen, Ching Kang ; Scotti, Anthony ; Simon, Melvin I. ; Hurley, James B. ; Slepak, Vladlen Z. / Autophosphorylation and ADP regulate the Ca2+-dependent interaction of recoverin with rhodopsin kinase. In: Biochemistry. 1998 ; Vol. 37, No. 28. pp. 10256-10262.
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