Autophagy is essential for maintaining the growth of a human (mini-)organ: Evidence from scalp hair follicle organ culture

Chiara Parodi, Jonathan A. Hardman, Giulia Allavena, Roberto Marotta, Tiziano Catelani, Marta Bertolini, Ralf Paus, Benedetto Grimaldi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Autophagy plays a crucial role in health and disease, regulating central cellular processes such as adaptive stress responses, differentiation, tissue development, and homeostasis. However, the role of autophagy in human physiology is poorly understood, highlighting a need for a model human organ system to assess the efficacy and safety of strategies to therapeutically modulate autophagy. As a complete, cyclically remodelled (mini-)organ, the organ culture of human scalp hair follicles (HFs), which, after massive growth (anagen), spontaneously enter into an apoptosis-driven organ involution (catagen) process, may provide such a model. Here, we reveal that in anagen, hair matrix keratinocytes (MKs) of organ-cultured HFs exhibit an active autophagic flux, as documented by evaluation of endogenous lipidated Light Chain 3B (LC3B) and sequestosome 1 (SQSTM1/p62) proteins and the ultrastructural visualization of autophagosomes at all stages of the autophagy process. This autophagic flux is altered during catagen, and genetic inhibition of autophagy promotes catagen development. Conversely, an anti-hair loss product markedly enhances intrafollicular autophagy, leading to anagen prolongation. Collectively, our data reveal a novel role of autophagy in human hair growth. Moreover, we show that organ-cultured scalp HFs are an excellent preclinical research model for exploring the role of autophagy in human tissue physiology and for evaluating the efficacy and tissue toxicity of candidate autophagy-modulatory agents in a living human (mini-)organ.

Original languageEnglish (US)
Pages (from-to)e2002864
JournalPLoS biology
Volume16
Issue number3
DOIs
StatePublished - Mar 1 2018

Fingerprint

hair follicles
organ culture
human growth
Hair Follicle
autophagy
Organ Culture Techniques
Autophagy
Scalp
hairs
Physiology
Tissue
Growth
Fluxes
Toxicity
Visualization
Health
Apoptosis
Hair
human physiology
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Parodi, C., Hardman, J. A., Allavena, G., Marotta, R., Catelani, T., Bertolini, M., ... Grimaldi, B. (2018). Autophagy is essential for maintaining the growth of a human (mini-)organ: Evidence from scalp hair follicle organ culture. PLoS biology, 16(3), e2002864. https://doi.org/10.1371/journal.pbio.2002864

Autophagy is essential for maintaining the growth of a human (mini-)organ : Evidence from scalp hair follicle organ culture. / Parodi, Chiara; Hardman, Jonathan A.; Allavena, Giulia; Marotta, Roberto; Catelani, Tiziano; Bertolini, Marta; Paus, Ralf; Grimaldi, Benedetto.

In: PLoS biology, Vol. 16, No. 3, 01.03.2018, p. e2002864.

Research output: Contribution to journalArticle

Parodi, C, Hardman, JA, Allavena, G, Marotta, R, Catelani, T, Bertolini, M, Paus, R & Grimaldi, B 2018, 'Autophagy is essential for maintaining the growth of a human (mini-)organ: Evidence from scalp hair follicle organ culture', PLoS biology, vol. 16, no. 3, pp. e2002864. https://doi.org/10.1371/journal.pbio.2002864
Parodi, Chiara ; Hardman, Jonathan A. ; Allavena, Giulia ; Marotta, Roberto ; Catelani, Tiziano ; Bertolini, Marta ; Paus, Ralf ; Grimaldi, Benedetto. / Autophagy is essential for maintaining the growth of a human (mini-)organ : Evidence from scalp hair follicle organ culture. In: PLoS biology. 2018 ; Vol. 16, No. 3. pp. e2002864.
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