TY - JOUR
T1 - Automated morphometric measurement of the retinal pigment epithelium complex and choriocapillaris using swept source OCT
AU - Zhou, Hao
AU - Dai, Yining
AU - Gregori, Giovanni
AU - Rosenfeld, Philip R.
AU - Duncan, Jacque L.
AU - Schwartz, Daniel M.
AU - Wang, Ruikang K.
N1 - Funding Information:
National Eye Institute (R01EY028753); Research to Prevent Blindness; Larry L. Hillblom Foundation; National Institutes of Health (EY002162); Foundation Fighting Blindness; Carl Zeiss Meditec, Inc; Claire Giannini Foundation; That Man May See, Inc..
Funding Information:
Dr. Gregori, Dr. Rosenfeld and Dr. Wang received research support from Carl Zeiss Meditec, Inc. Dr. Gregori and the University of Miami co-own a patent that is licensed to Carl Zeiss Meditec, Inc. Dr. Rosenfeld is a consultant for Apellis, Boehringer-Ingelheim, Carl Zeiss Meditec, Chengdu Kanghong Biotech, Ocunexus Therapeutics, Hemera Biosciences, F. Hoffmann-La Roche Ltd., Isarna Pharmaceuticals, Ocunexus, Ocudyne, and Unity Biotechnology. Dr. Rosenfeld has equity interest in Apellis, Verana Health, and Ocudyne. Dr. Duncan is a consultant for AGTC, California Institute for Regenerative Medicine, Foundation Fighting Blindness, Editas Medicine, Ionis Pharmaceuticals, Novelion Therapeutics, ProQR Therapeutics, SparingVision, Spark Therapeutics. Dr. Duncan also receives research support from Foundation Fighting Blindness, Neurotech USA, Inc. Dr. Wang discloses intellectual property owned by the Oregon Health and Science University and the University of Washington. Dr. Wang also receives research support from Tasso Inc, Moptim Inc, and Colgate Palmolive Company. He is a consultant to Insight Photonic Solutions and Carl Zeiss Meditec. Dr. Zhou, Dr. Dai and Dr. Schwartz declare no conflicts of interest.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - We report the development of an automated method to measure morphological features of the retinal pigment epithelium (RPE), Bruch's membrane (BM) and choriocapillaris (CC) using a commercially available swept source OCT (SS-OCT) system. The locations of the inner segment/outer segment (IS/OS), RPE and CC were determined by optical coherence tomography (OCT) and OCT angiography (OCTA) A-scan intensity profiles, which were used to calculate the mean IS/OS-to-RPE distance, mean RPE-to-CC distance, mean RPE-to-CC outer boundary distance, mean RPE thickness and mean CC thickness across the entire scan volume. The automated method was tested on two groups of normal subjects: younger age group (n=20, 30.3 ± 5.72 years, axial length = 24.2 ± 0.96 mm) and older age group (n=20, 80.8 ± 4.12 years, axial length = 23.5 ± 0.93 mm). The 6×6 mm macular scans were acquired from one eye of each subject. Repeatability of the measurements showed a coefficient of variance < 5% for all the cases. CC locations were confirmed qualitatively with pixel-by-pixel moving of the en face OCT/OCTA images. Relative distance and thickness maps of the RPE-BM-CC complex were generated for visualization of regional changes. We observed thinner CC, thinner RPE and increased RPE-to-CC distance in the older age group. Correlation between CC thickness and choroid thickness suggests that the CC thins with the overall thinning of the choroid. These metrics should be useful to reveal more morphological details of RPE-BM-CC complex, provide a better understanding of the CC in three dimensions, and further investigate potential functional relationships between RPE, BM and CC, and their involvement in age-related ocular diseases.
AB - We report the development of an automated method to measure morphological features of the retinal pigment epithelium (RPE), Bruch's membrane (BM) and choriocapillaris (CC) using a commercially available swept source OCT (SS-OCT) system. The locations of the inner segment/outer segment (IS/OS), RPE and CC were determined by optical coherence tomography (OCT) and OCT angiography (OCTA) A-scan intensity profiles, which were used to calculate the mean IS/OS-to-RPE distance, mean RPE-to-CC distance, mean RPE-to-CC outer boundary distance, mean RPE thickness and mean CC thickness across the entire scan volume. The automated method was tested on two groups of normal subjects: younger age group (n=20, 30.3 ± 5.72 years, axial length = 24.2 ± 0.96 mm) and older age group (n=20, 80.8 ± 4.12 years, axial length = 23.5 ± 0.93 mm). The 6×6 mm macular scans were acquired from one eye of each subject. Repeatability of the measurements showed a coefficient of variance < 5% for all the cases. CC locations were confirmed qualitatively with pixel-by-pixel moving of the en face OCT/OCTA images. Relative distance and thickness maps of the RPE-BM-CC complex were generated for visualization of regional changes. We observed thinner CC, thinner RPE and increased RPE-to-CC distance in the older age group. Correlation between CC thickness and choroid thickness suggests that the CC thins with the overall thinning of the choroid. These metrics should be useful to reveal more morphological details of RPE-BM-CC complex, provide a better understanding of the CC in three dimensions, and further investigate potential functional relationships between RPE, BM and CC, and their involvement in age-related ocular diseases.
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U2 - 10.1364/BOE.385113
DO - 10.1364/BOE.385113
M3 - Article
AN - SCOPUS:85082794765
VL - 11
SP - 1834
EP - 1850
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 4
ER -