Automated islet isolation from human pancreas

Camillo Ricordi, P. E. Lacy, D. W. Scharp

Research output: Contribution to journalArticle

288 Citations (Scopus)

Abstract

An automated method for the isolation of human pancreatic islets is described. The procedure meets the following requirements: 1) minimal traumatic action on the islets, 2) continuous digestion in which the islets that are progressively liberated can be saved from further enzymatic action, 3) minimal human intervention in the digestion process, and 4) high yield and purity of the isolated islets. After purification of Ficoll gradients, an average of 2279 islets/g pancreas was obtained, with an average purity of 79% islets. The average volume and average insulin content of the final islet preparation were 348 mm3 and 93.4 U, respectively. The islets were morphologically intact with a normal degree of β-cell granulation, responded to glucose stimulation with a fivefold increase of insulin secretion over basal levels, and produced normoglycemia after transplantation into diabetic mice. The procedure is being used in the second phase of clinical trials of islet transplantation in patients with insulin-dependent diabetes mellitus.

Original languageEnglish
Pages (from-to)140-142
Number of pages3
JournalDiabetes
Volume38
Issue numberSUPPL. 1
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Pancreas
Digestion
Insulin
Islets of Langerhans Transplantation
Ficoll
Type 1 Diabetes Mellitus
Islets of Langerhans
Transplantation
Clinical Trials
Glucose

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Ricordi, C., Lacy, P. E., & Scharp, D. W. (1989). Automated islet isolation from human pancreas. Diabetes, 38(SUPPL. 1), 140-142.

Automated islet isolation from human pancreas. / Ricordi, Camillo; Lacy, P. E.; Scharp, D. W.

In: Diabetes, Vol. 38, No. SUPPL. 1, 01.01.1989, p. 140-142.

Research output: Contribution to journalArticle

Ricordi, C, Lacy, PE & Scharp, DW 1989, 'Automated islet isolation from human pancreas', Diabetes, vol. 38, no. SUPPL. 1, pp. 140-142.
Ricordi C, Lacy PE, Scharp DW. Automated islet isolation from human pancreas. Diabetes. 1989 Jan 1;38(SUPPL. 1):140-142.
Ricordi, Camillo ; Lacy, P. E. ; Scharp, D. W. / Automated islet isolation from human pancreas. In: Diabetes. 1989 ; Vol. 38, No. SUPPL. 1. pp. 140-142.
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