Automated, high-throughput assays for evaluation of human pancreatic islet function

Over Cabrera, M. Caroline Jacques-Silva, Dora M. Berman, Alberto Fachado, Felipe Echeverri, Ramon Poo, Aisha Khan, Norma S. Kenyon, Camillo Ricordi, Per-Olof Ynge Berggren, Alejandro Caicedo

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

An important challenge in pancreatic islet transplantation in association with type 1 diabetes is to define automatic high-throughput assays for evaluation of human islet function. The physiological techniques presently used are amenable to small-scale experimental samples and produce descriptive results. The postgenomic era provides an opportunity to analyze biological processes on a larger scale, but the transition to high-throughput technologies is still a challenge. As a first step to implement high-throughput assays for the study of human islet function, we have developed two methodologies: multiple automated perifusion to determine islet hormone secretion and high-throughput kinetic imaging to examine islet cellular responses. Both technologies use fully automated devices that allow performing simultaneous experiments on multiple islet preparations. Our results illustrate that these technologies can be applied to study the functional status and explore the pharmacological profiles of islet cells. These methodologies will enable functional characterization of human islet preparations before transplantation and thereby provide the basis for the establishment of predictive tests for β-cell potency.

Original languageEnglish (US)
Pages (from-to)1039-1048
Number of pages10
JournalCell Transplantation
Volume16
Issue number10
DOIs
StatePublished - May 30 2008

Keywords

  • High-throughput assay
  • Human islet function
  • Pancreatic islets
  • Type 1 diabetes

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

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