Abstract
We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.
| Original language | English |
|---|---|
| Pages (from-to) | 157-161 |
| Number of pages | 5 |
| Journal | Clinical Biochemistry |
| Volume | 17 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1984 |
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Keywords
- centrifugal analyser
- Fibronectin
- nephelometry
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Automated fibronectin assay using light-scattering optics on a centrifugal analyzer. / Steele, Bernard W; Hills, Lorin P.; Hellman, Robert L.; Civantos, Francisco; Kent, Jessie.
In: Clinical Biochemistry, Vol. 17, No. 3, 01.01.1984, p. 157-161.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Automated fibronectin assay using light-scattering optics on a centrifugal analyzer
AU - Steele, Bernard W
AU - Hills, Lorin P.
AU - Hellman, Robert L.
AU - Civantos, Francisco
AU - Kent, Jessie
PY - 1984/1/1
Y1 - 1984/1/1
N2 - We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.
AB - We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.
KW - centrifugal analyser
KW - Fibronectin
KW - nephelometry
UR - http://www.scopus.com/inward/record.url?scp=0021144514&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021144514&partnerID=8YFLogxK
U2 - 10.1016/S0009-9120(84)80149-6
DO - 10.1016/S0009-9120(84)80149-6
M3 - Article
C2 - 6733895
AN - SCOPUS:0021144514
VL - 17
SP - 157
EP - 161
JO - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 3
ER -