Automated fibronectin assay using light-scattering optics on a centrifugal analyzer

Bernard W. Steele, Lorin P. Hills, Robert L. Hellman, Francisco Civantos, Jessie Kent

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.

Original languageEnglish (US)
Pages (from-to)157-161
Number of pages5
JournalClinical Biochemistry
Volume17
Issue number3
DOIs
StatePublished - Jun 1984

Keywords

  • centrifugal analyser
  • Fibronectin
  • nephelometry

ASJC Scopus subject areas

  • Clinical Biochemistry

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