Automated fibronectin assay using light-scattering optics on a centrifugal analyzer

Bernard W. Steele; Lorin P. Hills; Robert L. Hellman; Francisco Civantos; Jessie Kent

doi:10.1016/S0009-9120(84)80149-6

Automated fibronectin assay using light-scattering optics on a centrifugal analyzer

Bernard W. Steele, Lorin P. Hills, Robert L. Hellman, Francisco Civantos, Jessie Kent

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.

Original language	English (US)
Pages (from-to)	157-161
Number of pages	5
Journal	Clinical Biochemistry
Volume	17
Issue number	3
DOIs	https://doi.org/10.1016/S0009-9120(84)80149-6
State	Published - Jun 1984

Keywords

centrifugal analyser
Fibronectin
nephelometry

ASJC Scopus subject areas

Clinical Biochemistry

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10.1016/S0009-9120(84)80149-6

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Steele, B. W., Hills, L. P., Hellman, R. L., Civantos, F., & Kent, J. (1984). Automated fibronectin assay using light-scattering optics on a centrifugal analyzer. Clinical Biochemistry, 17(3), 157-161. https://doi.org/10.1016/S0009-9120(84)80149-6

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abstract = "We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.",

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AU - Kent, Jessie

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AB - We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.

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