Abstract
We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.
Original language | English (US) |
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Pages (from-to) | 157-161 |
Number of pages | 5 |
Journal | Clinical Biochemistry |
Volume | 17 |
Issue number | 3 |
DOIs | |
State | Published - Jun 1984 |
Keywords
- centrifugal analyser
- Fibronectin
- nephelometry
ASJC Scopus subject areas
- Clinical Biochemistry