Automated fibronectin assay using light-scattering optics on a centrifugal analyzer

Bernard W. Steele; Lorin P. Hills; Robert L. Hellman; Francisco Civantos; Jessie Kent

doi:10.1016/S0009-9120(84)80149-6

Automated fibronectin assay using light-scattering optics on a centrifugal analyzer

Bernard W. Steele, Lorin P. Hills, Robert L. Hellman, Francisco Civantos, Jessie Kent

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.

Original language	English (US)
Pages (from-to)	157-161
Number of pages	5
Journal	Clinical Biochemistry
Volume	17
Issue number	3
DOIs	https://doi.org/10.1016/S0009-9120(84)80149-6
State	Published - Jun 1984

Keywords

centrifugal analyser
Fibronectin
nephelometry

ASJC Scopus subject areas

Clinical Biochemistry

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10.1016/S0009-9120(84)80149-6

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title = "Automated fibronectin assay using light-scattering optics on a centrifugal analyzer",

abstract = "We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.",

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author = "Steele, {Bernard W.} and Hills, {Lorin P.} and Hellman, {Robert L.} and Francisco Civantos and Jessie Kent",

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AU - Steele, Bernard W.

AU - Hills, Lorin P.

AU - Hellman, Robert L.

AU - Civantos, Francisco

AU - Kent, Jessie

PY - 1984/6

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N2 - We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.

AB - We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.

KW - centrifugal analyser

KW - Fibronectin

KW - nephelometry

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