Automated fibronectin assay using light-scattering optics on a centrifugal analyzer

Bernard W Steele, Lorin P. Hills, Robert L. Hellman, Francisco Civantos, Jessie Kent

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We describe an automated, kinetic nephelometric method for fibronectin on the Multistat III F/LS Centrifugal Analyzer (Instrumentation Laboratory Inc., Lexington, MA 02173). Antiserum is diluted with polyethylene glycol. Calibrators and samples are prediluted with potassium phosphate buffer (10 mmol/L, pH 7.0) containing 8.5 g of NaCl per litre. Intensity (I) is read at 5 and 180 s and the resulting δI plotted against concentration. A non-linear least squares curve fitting and interpolation of results is carried out automatically. Three controls, with values of between 190-370 gave coefficients of variation between 4 and 7 percent, and the sensitivity of the method is to 25 mg/L.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalClinical Biochemistry
Volume17
Issue number3
DOIs
StatePublished - Jan 1 1984

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Curve fitting
Fibronectins
Light scattering
Immune Sera
Assays
Optics
Interpolation
Buffers
Light
Kinetics
Least-Squares Analysis
potassium phosphate

Keywords

  • centrifugal analyser
  • Fibronectin
  • nephelometry

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Automated fibronectin assay using light-scattering optics on a centrifugal analyzer. / Steele, Bernard W; Hills, Lorin P.; Hellman, Robert L.; Civantos, Francisco; Kent, Jessie.

In: Clinical Biochemistry, Vol. 17, No. 3, 01.01.1984, p. 157-161.

Research output: Contribution to journalArticle

Steele, Bernard W ; Hills, Lorin P. ; Hellman, Robert L. ; Civantos, Francisco ; Kent, Jessie. / Automated fibronectin assay using light-scattering optics on a centrifugal analyzer. In: Clinical Biochemistry. 1984 ; Vol. 17, No. 3. pp. 157-161.
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