TY - JOUR
T1 - Automated donor tissue preparation for Descemet Membrane Automated Endothelial Keratoplasty (DMAEK)
T2 - An experimental study
AU - Kymionis, George D.
AU - Yoo, Sonia H.
AU - Diakonis, Vasilios F.
AU - Grentzelos, Michael A.
AU - Naoumidi, Irini
AU - Pallikaris, Ioannis G.
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/3
Y1 - 2011/3
N2 - ■ BACKGROUND AND OBJECTIVE: Graft harvesting for Descemet membrane endothelial keratoplasty (DMEK) occurs manually and demonstrates surgical difficulties. In the current study, a mechanical automated technique for preparing a donor Descemet membrane (DM) carrying autologous endothelium for DMEK was evaluated. ■ MATERIALS AND METHODS: Ten rabbit corneas were placed on an artificial anterior chamber and mechanical separation of the DM was conducted using an Epi-keratome (Senturium; Norwood Abbey EyeCare, Victoria, Australia). All DM specimens were properly fixated and optical microscopy was performed. squf; RESULTS: DM separation was achieved in a controlled fashion in 7 of 10 eyes. As demonstrated by optical microscopy of the specimens, no corneal stroma was attached on the DM, whereas the endothelial layer was preserved in several areas. ■ CONCLUSION: Automated separation of the DM can be achieved using an Epi-keratome. Additional studies and improvements of the current technique are needed to draw final conclusions.
AB - ■ BACKGROUND AND OBJECTIVE: Graft harvesting for Descemet membrane endothelial keratoplasty (DMEK) occurs manually and demonstrates surgical difficulties. In the current study, a mechanical automated technique for preparing a donor Descemet membrane (DM) carrying autologous endothelium for DMEK was evaluated. ■ MATERIALS AND METHODS: Ten rabbit corneas were placed on an artificial anterior chamber and mechanical separation of the DM was conducted using an Epi-keratome (Senturium; Norwood Abbey EyeCare, Victoria, Australia). All DM specimens were properly fixated and optical microscopy was performed. squf; RESULTS: DM separation was achieved in a controlled fashion in 7 of 10 eyes. As demonstrated by optical microscopy of the specimens, no corneal stroma was attached on the DM, whereas the endothelial layer was preserved in several areas. ■ CONCLUSION: Automated separation of the DM can be achieved using an Epi-keratome. Additional studies and improvements of the current technique are needed to draw final conclusions.
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U2 - 10.3928/15428877-20101223-01
DO - 10.3928/15428877-20101223-01
M3 - Article
C2 - 21210581
AN - SCOPUS:79958143411
VL - 42
SP - 158
EP - 161
JO - Ophthalmic Surgery Lasers and Imaging Retina
JF - Ophthalmic Surgery Lasers and Imaging Retina
SN - 2325-8160
IS - 2
ER -