Autologous engineered cartilage rods for penile reconstruction

James J. Yoo, Heung Jae Park, Ilwoo Lee, Anthony Atala

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Purpose: Conditions such as inadequate and ambiguous genitalia that are caused by rudimentary penis, severe hypospadias or traumatic injury require surgical intervention. Although silicone penile prostheses are an accepted treatment modality, biocompatibility issues may be a problem in select cases. We previously demonstrated that rods composed of cartilage could be created using chondrocytes seeded on biodegradable polymer scaffolds. We showed that the cartilage rods engineered ex situ were readily elastic and withstood high degrees of pressure. We investigated the feasibility of applying the engineered cartilage rods in situ in an animal model. Materials and Methods: Autologous chondrocytes harvested from rabbit ears were grown and expanded in culture. Cells were seeded onto biodegradable poly-L-lactic acid coated polyglycolic acid polymer rods at a concentration of 50 x 106 chondrocytes per cm. A total of 18 chondrocyte polymer scaffolds were implanted into the corporal spaces in 10 rabbits. As controls, 1 corpus in each of 2 rabbits was not implanted. The animals were sacrificed 1, 2, 3 or 6 months after implantation. Histological analysis was performed using hematoxylin and eosin, aldehyde fuschin-alcian blue and toluidine blue staining. Results: All animals tolerated the implants for the duration of the study without any complications. Gross examination after retrieval at i month showed well formed, milky white cartilage structures within the corpora. All polymers were fully degraded by 2 months. There was no evidence of erosion or infection at any of the implant sites. Histological analysis using alcian blue and toluidine blue staining revealed mature and well formed chondrocytes in the retrieved implants. Conclusions: Autologous chondrocytes seeded on preformed biodegradable polymer structures form cartilage structures within the rabbit corpus cavernosum. This technology appears to be useful for creating autologous penile prostheses.

Original languageEnglish
Pages (from-to)1119-1121
Number of pages3
JournalJournal of Urology
Volume162
Issue number3 II
DOIs
StatePublished - Sep 1 1999
Externally publishedYes

Fingerprint

Chondrocytes
Cartilage
Polymers
Penile Prosthesis
Rabbits
Tolonium Chloride
Alcian Blue
Staining and Labeling
Polyglycolic Acid
Disorders of Sex Development
Hypospadias
Intraoperative Complications
Penis
Silicones
Hematoxylin
Eosine Yellowish-(YS)
Aldehydes
Ear
Animal Models
Technology

Keywords

  • Cartilage
  • Chondrocytes
  • Penis
  • Prostheses and implants

ASJC Scopus subject areas

  • Urology

Cite this

Autologous engineered cartilage rods for penile reconstruction. / Yoo, James J.; Park, Heung Jae; Lee, Ilwoo; Atala, Anthony.

In: Journal of Urology, Vol. 162, No. 3 II, 01.09.1999, p. 1119-1121.

Research output: Contribution to journalArticle

Yoo, JJ, Park, HJ, Lee, I & Atala, A 1999, 'Autologous engineered cartilage rods for penile reconstruction', Journal of Urology, vol. 162, no. 3 II, pp. 1119-1121. https://doi.org/10.1016/S0022-5347(01)68090-X
Yoo, James J. ; Park, Heung Jae ; Lee, Ilwoo ; Atala, Anthony. / Autologous engineered cartilage rods for penile reconstruction. In: Journal of Urology. 1999 ; Vol. 162, No. 3 II. pp. 1119-1121.
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