Autoinducers extracted from microbial mats reveal a surprising diversity of N-acylhomoserine lactones (AHLs) and abundance changes that may relate to diel pH

Alan W. Decho, Pieter T. Visscher, John Ferry, Tomohiro Kawaguchi, Lijian He, Kristen M. Przekop, R. Sean Norman, Pamela R Reid

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Microbial mats are highly structured and diverse communities, and one of the earliest-known life assemblages. Mat bacteria interact within an environment marked by strong geochemical gradients and fluctuations. We examined natural mat systems for the presence of autoinducers involved in quorum sensing, a form of cell-cell communication. Our results revealed that a diverse array of N-acylhomoserine lactones (AHLs) including C4- to C 14-AHLs, were identified from mat extracts using mass spectrometry (MS), with further confirmation by MS/MS-collision-induced dissociation (CID), and additions of external standards. Microelectrode measurements showed that mats exhibited diel pH fluctuations, ranging from alkaline (pH 9.4) during daytime (net photosynthesis) to acidic (pH 6.8) during darkness (net respiration/fermentation). Under laboratory conditions, AHLs having shorter acyl-chains were degraded within the time frame that daily alkaline pH (> 8.2) conditions exist in mats. Intensive sampling of mats after full day- or night-time incubations revealed that accumulations of extractable shorter-chain AHLs (e.g. C8- and C10-AHLs) were significantly (P < 0.001) diminished during daytime. Our study offers evidence that stabilities of AHLs under natural conditions may be influenced by the proximal extracellular environment. We further propose that the ancient periodicity of photosynthesis/respiration in mats may potentially drive a mechanism for diel differences in activities of certain autoinducers, and hence bacterial activities mediated through quorum sensing.

Original languageEnglish (US)
Pages (from-to)409-420
Number of pages12
JournalEnvironmental Microbiology
Volume11
Issue number2
DOIs
StatePublished - Feb 2009

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microbial mat
Lactones
lactones
mass spectrometry
photosynthesis
respiration
Quorum Sensing
quorum sensing
Photosynthesis
cell respiration
microbial activity
periodicity
fermentation
Respiration
collision
incubation
communication
cell communication
bacterium
Darkness

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Autoinducers extracted from microbial mats reveal a surprising diversity of N-acylhomoserine lactones (AHLs) and abundance changes that may relate to diel pH. / Decho, Alan W.; Visscher, Pieter T.; Ferry, John; Kawaguchi, Tomohiro; He, Lijian; Przekop, Kristen M.; Norman, R. Sean; Reid, Pamela R.

In: Environmental Microbiology, Vol. 11, No. 2, 02.2009, p. 409-420.

Research output: Contribution to journalArticle

Decho, Alan W. ; Visscher, Pieter T. ; Ferry, John ; Kawaguchi, Tomohiro ; He, Lijian ; Przekop, Kristen M. ; Norman, R. Sean ; Reid, Pamela R. / Autoinducers extracted from microbial mats reveal a surprising diversity of N-acylhomoserine lactones (AHLs) and abundance changes that may relate to diel pH. In: Environmental Microbiology. 2009 ; Vol. 11, No. 2. pp. 409-420.
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