Autocrine growth induced by src-related oncogenes in transformed chicken myeloid cells

Becky Adkins, Achim Leutz, Thomas Graf

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

Chicken myeloid cells transformed by the v-myb- or v-myc-containing leukemia viruses, E26 and OK10, respectively, require chicken myelomonocytic growth factor (cMGF) for proliferation in vitro. Upon superinfection with retroviruses carrying oncogenes of the src gene family, these myeloid cells acquire the ability to grow in the absence of exogenous cMGF. Conditioned medium prepared from superinfected E26 cells contains a growth-stimulating activity similar in biological and immunological properties to cMGF. This activity is reduced by more than 80% following absorption of conditioned media with antiserum against cMGF. Incubation of superinfected E26 cells with an immunoglobulin fraction of antiserum against cMGF inhibits their proliferation, indicating that the cells are dependent on the secreted factor. We conclude that viral oncogenes of the src family can induce chicken myeloid cells to produce a cMGF-like factor(s) that stimulates proliferation of these cells in an autocrine fashion.

Original languageEnglish (US)
Pages (from-to)439-445
Number of pages7
JournalCell
Volume39
Issue number3 PART 2
DOIs
StatePublished - Dec 1984

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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