Autocrine and paracrine roles for ATP and serotonin in mouse taste buds

Yijen A. Huang, Robin Dando, Stephen D. Roper

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

Receptor (type II) taste bud cells secrete ATP during taste stimulation. In turn, ATP activates adjacent presynaptic (type III) cells to release serotonin (5-hydroxytryptamine, or 5-HT) and norepinephrine (NE). The roles of these neurotransmitters in taste buds have not been fully elucidated. Here we tested whether ATP or 5-HT exert feedback onto receptor (type II) cells during taste stimulation. Our previous studies showed NE does not appear to act on adjacent taste bud cells, or at least on receptor cells. Our data show that 5-HT released from presynaptic (type III) cells provides negative paracrine feedback onto receptor cells by activating 5-HT1A receptors, inhibiting taste-evoked Ca2+ mobilization in receptor cells, and reducing ATP secretion. The findings also demonstrate that ATP exerts positive autocrine feedback onto receptor (type II) cells by activating P2Y1 receptors and enhancing ATP secretion. These results begin to sort out how purinergic and aminergic transmitters function within the taste bud to modulate gustatory signaling in these peripheral sensory organs.

Original languageEnglish (US)
Pages (from-to)13909-13918
Number of pages10
JournalJournal of Neuroscience
Volume29
Issue number44
DOIs
StatePublished - Nov 4 2009

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint

Dive into the research topics of 'Autocrine and paracrine roles for ATP and serotonin in mouse taste buds'. Together they form a unique fingerprint.

Cite this