Auromomycin-induced DNA damage and repair in human leukemic lymphoblasts (CCRF-CEM cells)

T. S A Samy, L. Wellham, A. Krishan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An alkaline elution procedure was used to study the nature of DNA damage induced by auromomycin, an antitumor protein, in human leukemic lymphoblasts (CCRF-CEM cells). The filter elution of drug-treated cells at pH 12.2 and 9.6 showed induction of both single and double strand DNA breaks. The DNA strand scission activities were linear in relation to drug concentration. The frequency of single strand breaks was higher than that of the double strand breaks. Protein-associated DNA single strand breaks were also detected in alkaline elution of drug-treated cells when a proteinase K digestion step was included in the assay protocol. The auromomycin-induced single strand breaks were repaired to almost completion within 8 h of postincubation of DNA-damaged cells whereas the repair of double strand breaks was not detected.

Original languageEnglish
Pages (from-to)507-512
Number of pages6
JournalCancer Research
Volume46
Issue number2
StatePublished - Jan 1 1986
Externally publishedYes

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DNA Repair
DNA Damage
Single-Stranded DNA Breaks
Pharmaceutical Preparations
Endopeptidase K
Double-Stranded DNA Breaks
DNA
Digestion
Proteins
auromomycin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Samy, T. S. A., Wellham, L., & Krishan, A. (1986). Auromomycin-induced DNA damage and repair in human leukemic lymphoblasts (CCRF-CEM cells). Cancer Research, 46(2), 507-512.

Auromomycin-induced DNA damage and repair in human leukemic lymphoblasts (CCRF-CEM cells). / Samy, T. S A; Wellham, L.; Krishan, A.

In: Cancer Research, Vol. 46, No. 2, 01.01.1986, p. 507-512.

Research output: Contribution to journalArticle

Samy, TSA, Wellham, L & Krishan, A 1986, 'Auromomycin-induced DNA damage and repair in human leukemic lymphoblasts (CCRF-CEM cells)', Cancer Research, vol. 46, no. 2, pp. 507-512.
Samy, T. S A ; Wellham, L. ; Krishan, A. / Auromomycin-induced DNA damage and repair in human leukemic lymphoblasts (CCRF-CEM cells). In: Cancer Research. 1986 ; Vol. 46, No. 2. pp. 507-512.
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