Augmentation of ADCC in mice by microbial fractions

Stephen P Richman, V. T. Chism, S. G. Murphy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effects of a multicomponent microbial vaccine on murine (BALB/c) splenocyte antibody-dependent cytotoxicity (ADCC) to chicken red blood cells were studied. The vaccine consisted of BCG cell wall skeleton (CWS), trehalose dimycolate, and mutant S. typhimurium endotoxin attached to mineral oil microdroplets, and was administered in a single intravenous dose. ADCC began to increase as early as 2 days following injection, was significant by 5 days, persisted for as long as 20 days, and was associated with splenic hypertrophy. Administration of the vaccine over a dose range of 1.5-15-150 μg demonstrated an increasing cytotoxic response with increasing dosage. The majority of cytotoxic activity was eliminated by passage of the effector cells through Sephadex G-10. Serial addition of each of the three components to the preparation and testing of each of the components separately revealed that no one component was responsible for the augmentation of the ADCC, but that the CWS probably made the least contribution. Subtraction of the oil from the whole vaccine preparation resulted in a modest but significant loss of augmenting activity.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalCancer Immunology Immunotherapy
Volume11
Issue number3
DOIs
StatePublished - Aug 1 1981
Externally publishedYes

Fingerprint

Cell Wall Skeleton
Vaccines
Antibodies
Cord Factors
BCG Vaccine
Mineral Oil
Endotoxins
Hypertrophy
Chickens
Oils
Erythrocytes
Injections

ASJC Scopus subject areas

  • Oncology
  • Immunology
  • Cancer Research

Cite this

Augmentation of ADCC in mice by microbial fractions. / Richman, Stephen P; Chism, V. T.; Murphy, S. G.

In: Cancer Immunology Immunotherapy, Vol. 11, No. 3, 01.08.1981, p. 233-238.

Research output: Contribution to journalArticle

Richman, Stephen P ; Chism, V. T. ; Murphy, S. G. / Augmentation of ADCC in mice by microbial fractions. In: Cancer Immunology Immunotherapy. 1981 ; Vol. 11, No. 3. pp. 233-238.
@article{1106ecda10c749c486159ac645d9706f,
title = "Augmentation of ADCC in mice by microbial fractions",
abstract = "The effects of a multicomponent microbial vaccine on murine (BALB/c) splenocyte antibody-dependent cytotoxicity (ADCC) to chicken red blood cells were studied. The vaccine consisted of BCG cell wall skeleton (CWS), trehalose dimycolate, and mutant S. typhimurium endotoxin attached to mineral oil microdroplets, and was administered in a single intravenous dose. ADCC began to increase as early as 2 days following injection, was significant by 5 days, persisted for as long as 20 days, and was associated with splenic hypertrophy. Administration of the vaccine over a dose range of 1.5-15-150 μg demonstrated an increasing cytotoxic response with increasing dosage. The majority of cytotoxic activity was eliminated by passage of the effector cells through Sephadex G-10. Serial addition of each of the three components to the preparation and testing of each of the components separately revealed that no one component was responsible for the augmentation of the ADCC, but that the CWS probably made the least contribution. Subtraction of the oil from the whole vaccine preparation resulted in a modest but significant loss of augmenting activity.",
author = "Richman, {Stephen P} and Chism, {V. T.} and Murphy, {S. G.}",
year = "1981",
month = "8",
day = "1",
doi = "10.1007/BF00199496",
language = "English",
volume = "11",
pages = "233--238",
journal = "Cancer Immunology and Immunotherapy",
issn = "0340-7004",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "3",

}

TY - JOUR

T1 - Augmentation of ADCC in mice by microbial fractions

AU - Richman, Stephen P

AU - Chism, V. T.

AU - Murphy, S. G.

PY - 1981/8/1

Y1 - 1981/8/1

N2 - The effects of a multicomponent microbial vaccine on murine (BALB/c) splenocyte antibody-dependent cytotoxicity (ADCC) to chicken red blood cells were studied. The vaccine consisted of BCG cell wall skeleton (CWS), trehalose dimycolate, and mutant S. typhimurium endotoxin attached to mineral oil microdroplets, and was administered in a single intravenous dose. ADCC began to increase as early as 2 days following injection, was significant by 5 days, persisted for as long as 20 days, and was associated with splenic hypertrophy. Administration of the vaccine over a dose range of 1.5-15-150 μg demonstrated an increasing cytotoxic response with increasing dosage. The majority of cytotoxic activity was eliminated by passage of the effector cells through Sephadex G-10. Serial addition of each of the three components to the preparation and testing of each of the components separately revealed that no one component was responsible for the augmentation of the ADCC, but that the CWS probably made the least contribution. Subtraction of the oil from the whole vaccine preparation resulted in a modest but significant loss of augmenting activity.

AB - The effects of a multicomponent microbial vaccine on murine (BALB/c) splenocyte antibody-dependent cytotoxicity (ADCC) to chicken red blood cells were studied. The vaccine consisted of BCG cell wall skeleton (CWS), trehalose dimycolate, and mutant S. typhimurium endotoxin attached to mineral oil microdroplets, and was administered in a single intravenous dose. ADCC began to increase as early as 2 days following injection, was significant by 5 days, persisted for as long as 20 days, and was associated with splenic hypertrophy. Administration of the vaccine over a dose range of 1.5-15-150 μg demonstrated an increasing cytotoxic response with increasing dosage. The majority of cytotoxic activity was eliminated by passage of the effector cells through Sephadex G-10. Serial addition of each of the three components to the preparation and testing of each of the components separately revealed that no one component was responsible for the augmentation of the ADCC, but that the CWS probably made the least contribution. Subtraction of the oil from the whole vaccine preparation resulted in a modest but significant loss of augmenting activity.

UR - http://www.scopus.com/inward/record.url?scp=0019490634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019490634&partnerID=8YFLogxK

U2 - 10.1007/BF00199496

DO - 10.1007/BF00199496

M3 - Article

VL - 11

SP - 233

EP - 238

JO - Cancer Immunology and Immunotherapy

JF - Cancer Immunology and Immunotherapy

SN - 0340-7004

IS - 3

ER -