Attempts to define functional domains of gap junction proteins with synthetic peptides

G. Dahl, Wolfgang F. Nonner, R. Werner

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

To map the binding sites involved in channel formation, synthetic peptides representing sequences of connexin32 were tested for their ability to inhibit cell-cell channel formation. Both large peptides representing most of the two presumed extracellular loops of connexin32 and shorter peptides representing subsets of these larger peptides were found to inhibit cell-cell channel formation. The properties of the peptide inhibition suggested that the binding site is complex, involving several segments of both extracellular loops. One of the peptides (a 12-mer) did not inhibit but instead was found to form channels in membranes. Both in oocyte membranes and in bilayers, the channels formed by the peptide were asymmetrically voltage dependent. Their unit conductances ranged from 20 to 160 pS. These data are discussed in the form of a model in which the connexin sequence represented by the peptide is part of a β structure providing the lining of the channel pore.

Original languageEnglish (US)
Pages (from-to)1816-1822
Number of pages7
JournalBiophysical journal
Volume67
Issue number5
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Biophysics

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