Atrial natriuretic peptide in acute hypoxia-induced pulmonary hypertension in rats

H. Jin, R. H. Yang, Y. F. Chen, Robert Jackson, H. Itoh, M. Mukoyama, K. Nakao, H. Imura, S. Oparil

Research output: Contribution to journalArticle

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Abstract

To test the hypothesis that exogenous atrial natriuretic peptide (ANP) prevents the acute pulmonary pressor response to hypoxia, ANP (20-μg/kg bolus followed by 1-μg·kg-1·min-1 infusion) or vehicle was administered intravenously to conscious rats beginning 3 min before exposure to hypoxia or room air for 90 min. Exogenous ANP abolished the acute pulmonary pressor response to hypoxia in association with marked and parallel increases in plasma ANP and guanosine 5'-cyclic monophosphate (cGMP) and with a significant increase in lung cGMP content. To examine whether endogenous ANP modulates the acute pulmonary pressor response to hypoxia, rats were pretreated with a monoclonal antibody (Ab) to ANP and exposed to hypoxia. Mean pulmonary arterial pressure (MPAP) in the Ab-treated rats was not different from control over the first 6 h of hypoxic exposure. Thereafter, the Ab-treated group had significantly higher MPAP than control. Our data suggest that 1) exogenous ANP blocks the pulmonary pressor response to acute hypoxia via stimulation of cGMP accumulation in the pulmonary vasculature, and 2) endogenous ANP may modulate the subacute, but not acute, phase of hypoxic pulmonary hypertension.

Original languageEnglish
Pages (from-to)807-814
Number of pages8
JournalJournal of Applied Physiology
Volume71
Issue number3
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor
Pulmonary Hypertension
Lung
Arterial Pressure
Guanosine Monophosphate
Hypoxia
Antibodies
Cyclic GMP
Air
Monoclonal Antibodies

Keywords

  • guanosine 5'-cyclic monophosphate
  • monoclonal antibody
  • pulmonary arterial pressure

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Jin, H., Yang, R. H., Chen, Y. F., Jackson, R., Itoh, H., Mukoyama, M., ... Oparil, S. (1991). Atrial natriuretic peptide in acute hypoxia-induced pulmonary hypertension in rats. Journal of Applied Physiology, 71(3), 807-814.

Atrial natriuretic peptide in acute hypoxia-induced pulmonary hypertension in rats. / Jin, H.; Yang, R. H.; Chen, Y. F.; Jackson, Robert; Itoh, H.; Mukoyama, M.; Nakao, K.; Imura, H.; Oparil, S.

In: Journal of Applied Physiology, Vol. 71, No. 3, 01.01.1991, p. 807-814.

Research output: Contribution to journalArticle

Jin, H, Yang, RH, Chen, YF, Jackson, R, Itoh, H, Mukoyama, M, Nakao, K, Imura, H & Oparil, S 1991, 'Atrial natriuretic peptide in acute hypoxia-induced pulmonary hypertension in rats', Journal of Applied Physiology, vol. 71, no. 3, pp. 807-814.
Jin, H. ; Yang, R. H. ; Chen, Y. F. ; Jackson, Robert ; Itoh, H. ; Mukoyama, M. ; Nakao, K. ; Imura, H. ; Oparil, S. / Atrial natriuretic peptide in acute hypoxia-induced pulmonary hypertension in rats. In: Journal of Applied Physiology. 1991 ; Vol. 71, No. 3. pp. 807-814.
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