Atp10p Assists Assembly of Atp6p into the F0 Unit of the Yeast Mitochondrial ATPase

Alexander Tzagoloff, Antonio Barrientos, Walter Neupert, Johannes M. Herrmann

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The F0F1-ATPase complex of yeast mitochondria contains three mitochondrial and at least 17 nuclear gene products. The coordinate assembly of mitochondrial and cytosolic translation products relies on chaperones and specific factors that stabilize the pools of some unassembled subunits. Atp10p was identified as a mitochondrial inner membrane component necessary for the biogenesis of the hydrophobic F0 sector of the ATPase. Here we show that, following its synthesis on mitochondrial ribosomes, subunit 6 of the ATPase (Atp6p) can be cross-linked to Atp10p. This interaction is required for the integration of Atp6p into a partially assembled subcomplex of the ATPase. Pulse labeling and chase of mitochondrial translation products in vivo indicate that Atp6p is less stable and more rapidly degraded in an atp10 null mutant than in wild type. Based on these observations, we propose Atp10p to be an Atp6p-specific chaperone that facilitates the incorporation of Atp6p into an intermediate subcomplex of ATPase subunits.

Original languageEnglish
Pages (from-to)19775-19780
Number of pages6
JournalJournal of Biological Chemistry
Volume279
Issue number19
DOIs
StatePublished - May 7 2004

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Yeast
Adenosine Triphosphatases
Proton-Translocating ATPases
Yeasts
Ribosome Subunits
Mitochondrial Membranes
Mitochondria
Labeling
Genes
Membranes
Mitochondrial Ribosomes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Atp10p Assists Assembly of Atp6p into the F0 Unit of the Yeast Mitochondrial ATPase. / Tzagoloff, Alexander; Barrientos, Antonio; Neupert, Walter; Herrmann, Johannes M.

In: Journal of Biological Chemistry, Vol. 279, No. 19, 07.05.2004, p. 19775-19780.

Research output: Contribution to journalArticle

Tzagoloff, Alexander ; Barrientos, Antonio ; Neupert, Walter ; Herrmann, Johannes M. / Atp10p Assists Assembly of Atp6p into the F0 Unit of the Yeast Mitochondrial ATPase. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 19. pp. 19775-19780.
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