ATP stimulates calcium influx in primary astrocyte cultures

J. T. Neary, C. van Breemen, E. Forster, L. O B Norenberg, Michael D Norenberg

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The effect of ATP and other purines on 45Ca uptake was studied in primary cultures of rat astrocytes. Treatment of the cells with ATP for 1 to 30 min brought about an increase in cellular 45Ca. Stimulation of calcium influx by ATP was investigated using a 90 sec exposure to 45Ca and over a concentration range of 0.1 nM to 3 mM; a biphasic dose-response curve was obtained with EC50 values of 0.3 nM and 9 uM, indicating the presence of low and high affinity purinergic binding sites. Similar levels of 45Ca influx at 90 sec were observed with ATP, ADP and adenosine (all at 100 uM). Prior treatment of the cultures with LaCl3 blocked the purine-induced 45Ca influx. These findings indicate that one pathway for calcium entry in astrocytes involves purinergic receptor-operated, calcium channels.

Original languageEnglish
Pages (from-to)1410-1416
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume157
Issue number3
DOIs
StatePublished - Dec 30 1988
Externally publishedYes

Fingerprint

Astrocytes
Adenosine Triphosphate
Calcium
Purinergic Receptors
Purines
Calcium Channels
Cell culture
Adenosine
Adenosine Diphosphate
Rats
Binding Sites
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

ATP stimulates calcium influx in primary astrocyte cultures. / Neary, J. T.; van Breemen, C.; Forster, E.; Norenberg, L. O B; Norenberg, Michael D.

In: Biochemical and Biophysical Research Communications, Vol. 157, No. 3, 30.12.1988, p. 1410-1416.

Research output: Contribution to journalArticle

Neary, J. T. ; van Breemen, C. ; Forster, E. ; Norenberg, L. O B ; Norenberg, Michael D. / ATP stimulates calcium influx in primary astrocyte cultures. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 157, No. 3. pp. 1410-1416.
@article{e8c6988ffb2b49e69fe1b8f925e6d851,
title = "ATP stimulates calcium influx in primary astrocyte cultures",
abstract = "The effect of ATP and other purines on 45Ca uptake was studied in primary cultures of rat astrocytes. Treatment of the cells with ATP for 1 to 30 min brought about an increase in cellular 45Ca. Stimulation of calcium influx by ATP was investigated using a 90 sec exposure to 45Ca and over a concentration range of 0.1 nM to 3 mM; a biphasic dose-response curve was obtained with EC50 values of 0.3 nM and 9 uM, indicating the presence of low and high affinity purinergic binding sites. Similar levels of 45Ca influx at 90 sec were observed with ATP, ADP and adenosine (all at 100 uM). Prior treatment of the cultures with LaCl3 blocked the purine-induced 45Ca influx. These findings indicate that one pathway for calcium entry in astrocytes involves purinergic receptor-operated, calcium channels.",
author = "Neary, {J. T.} and {van Breemen}, C. and E. Forster and Norenberg, {L. O B} and Norenberg, {Michael D}",
year = "1988",
month = "12",
day = "30",
doi = "10.1016/S0006-291X(88)81032-5",
language = "English",
volume = "157",
pages = "1410--1416",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - ATP stimulates calcium influx in primary astrocyte cultures

AU - Neary, J. T.

AU - van Breemen, C.

AU - Forster, E.

AU - Norenberg, L. O B

AU - Norenberg, Michael D

PY - 1988/12/30

Y1 - 1988/12/30

N2 - The effect of ATP and other purines on 45Ca uptake was studied in primary cultures of rat astrocytes. Treatment of the cells with ATP for 1 to 30 min brought about an increase in cellular 45Ca. Stimulation of calcium influx by ATP was investigated using a 90 sec exposure to 45Ca and over a concentration range of 0.1 nM to 3 mM; a biphasic dose-response curve was obtained with EC50 values of 0.3 nM and 9 uM, indicating the presence of low and high affinity purinergic binding sites. Similar levels of 45Ca influx at 90 sec were observed with ATP, ADP and adenosine (all at 100 uM). Prior treatment of the cultures with LaCl3 blocked the purine-induced 45Ca influx. These findings indicate that one pathway for calcium entry in astrocytes involves purinergic receptor-operated, calcium channels.

AB - The effect of ATP and other purines on 45Ca uptake was studied in primary cultures of rat astrocytes. Treatment of the cells with ATP for 1 to 30 min brought about an increase in cellular 45Ca. Stimulation of calcium influx by ATP was investigated using a 90 sec exposure to 45Ca and over a concentration range of 0.1 nM to 3 mM; a biphasic dose-response curve was obtained with EC50 values of 0.3 nM and 9 uM, indicating the presence of low and high affinity purinergic binding sites. Similar levels of 45Ca influx at 90 sec were observed with ATP, ADP and adenosine (all at 100 uM). Prior treatment of the cultures with LaCl3 blocked the purine-induced 45Ca influx. These findings indicate that one pathway for calcium entry in astrocytes involves purinergic receptor-operated, calcium channels.

UR - http://www.scopus.com/inward/record.url?scp=0024263304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024263304&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(88)81032-5

DO - 10.1016/S0006-291X(88)81032-5

M3 - Article

VL - 157

SP - 1410

EP - 1416

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -