ATP stimulates calcium influx in primary astrocyte cultures

J. T. Neary, C. van Breemen, E. Forster, L. O.B. Norenberg, M. D. Norenberg

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

The effect of ATP and other purines on 45Ca uptake was studied in primary cultures of rat astrocytes. Treatment of the cells with ATP for 1 to 30 min brought about an increase in cellular 45Ca. Stimulation of calcium influx by ATP was investigated using a 90 sec exposure to 45Ca and over a concentration range of 0.1 nM to 3 mM; a biphasic dose-response curve was obtained with EC50 values of 0.3 nM and 9 uM, indicating the presence of low and high affinity purinergic binding sites. Similar levels of 45Ca influx at 90 sec were observed with ATP, ADP and adenosine (all at 100 uM). Prior treatment of the cultures with LaCl3 blocked the purine-induced 45Ca influx. These findings indicate that one pathway for calcium entry in astrocytes involves purinergic receptor-operated, calcium channels.

Original languageEnglish (US)
Pages (from-to)1410-1416
Number of pages7
JournalBiochemical and biophysical research communications
Volume157
Issue number3
DOIs
StatePublished - Dec 30 1988

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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