ATP-sensitive potassium (KATP) currents were examined in dorsal root ganglion neurons from neuropathic and control rats using whole-cell voltage clamp recordings. KATP channel openers (diazoxide and pinacidil) enhanced, and the blocker glibenclamide inhibited an outward current in control neurons in a manner dependent on the pipette ATP concentration. Analysis of reversal potentials showed that this current is carried by K + ions. Outward current in cells from rats with peripheral nerve injury was not sensitive to modulators of KATP channels. Gabapentin, a putative KATP channel opener, had minimal effect on currents in either group of neurons. We conclude that normal primary afferent neurons express KATP channels that conduct current which is eliminated by peripheral nerve injury. Gabapentin does not affect this current significantly.
- ATP-sensitive potassium channels
- Chronic constriction injury
- Primary afferent neurons
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