ATP-sensitive potassium channels in rat primary afferent neurons: The effect of neuropathic injury and gabapentin

Constantine Sarantopoulos; Bruce McCallum; Damir Sapunar; Wai Meng Kwok; Quinn Hogan

doi:10.1016/S0304-3940(03)00383-5

ATP-sensitive potassium channels in rat primary afferent neurons: The effect of neuropathic injury and gabapentin

Constantine Sarantopoulos, Bruce McCallum, Damir Sapunar, Wai Meng Kwok, Quinn Hogan

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

ATP-sensitive potassium (K_ATP) currents were examined in dorsal root ganglion neurons from neuropathic and control rats using whole-cell voltage clamp recordings. K_ATP channel openers (diazoxide and pinacidil) enhanced, and the blocker glibenclamide inhibited an outward current in control neurons in a manner dependent on the pipette ATP concentration. Analysis of reversal potentials showed that this current is carried by K ⁺ ions. Outward current in cells from rats with peripheral nerve injury was not sensitive to modulators of K_ATP channels. Gabapentin, a putative K_ATP channel opener, had minimal effect on currents in either group of neurons. We conclude that normal primary afferent neurons express K_ATP channels that conduct current which is eliminated by peripheral nerve injury. Gabapentin does not affect this current significantly.

Original language	English (US)
Pages (from-to)	185-189
Number of pages	5
Journal	Neuroscience Letters
Volume	343
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(03)00383-5
State	Published - Jun 12 2003
Externally published	Yes

Keywords

ATP-sensitive potassium channels
Chronic constriction injury
Diazoxide
Gabapentin
Glibenclamide
Neuropathy
Pinacidil
Primary afferent neurons

ASJC Scopus subject areas

Neuroscience(all)

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title = "ATP-sensitive potassium channels in rat primary afferent neurons: The effect of neuropathic injury and gabapentin",

abstract = "ATP-sensitive potassium (KATP) currents were examined in dorsal root ganglion neurons from neuropathic and control rats using whole-cell voltage clamp recordings. KATP channel openers (diazoxide and pinacidil) enhanced, and the blocker glibenclamide inhibited an outward current in control neurons in a manner dependent on the pipette ATP concentration. Analysis of reversal potentials showed that this current is carried by K + ions. Outward current in cells from rats with peripheral nerve injury was not sensitive to modulators of KATP channels. Gabapentin, a putative KATP channel opener, had minimal effect on currents in either group of neurons. We conclude that normal primary afferent neurons express KATP channels that conduct current which is eliminated by peripheral nerve injury. Gabapentin does not affect this current significantly.",

keywords = "ATP-sensitive potassium channels, Chronic constriction injury, Diazoxide, Gabapentin, Glibenclamide, Neuropathy, Pinacidil, Primary afferent neurons",

author = "Constantine Sarantopoulos and Bruce McCallum and Damir Sapunar and Kwok, {Wai Meng} and Quinn Hogan",

note = "Funding Information: This work was supported by the Foundation for Anesthesia Education and Research (CS) and NIH grant NS RO1-42150 (QH). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2003",

month = jun,

day = "12",

doi = "10.1016/S0304-3940(03)00383-5",

language = "English (US)",

volume = "343",

pages = "185--189",

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T1 - ATP-sensitive potassium channels in rat primary afferent neurons

T2 - The effect of neuropathic injury and gabapentin

AU - Sarantopoulos, Constantine

AU - McCallum, Bruce

AU - Sapunar, Damir

AU - Kwok, Wai Meng

AU - Hogan, Quinn

N1 - Funding Information: This work was supported by the Foundation for Anesthesia Education and Research (CS) and NIH grant NS RO1-42150 (QH). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2003/6/12

Y1 - 2003/6/12

N2 - ATP-sensitive potassium (KATP) currents were examined in dorsal root ganglion neurons from neuropathic and control rats using whole-cell voltage clamp recordings. KATP channel openers (diazoxide and pinacidil) enhanced, and the blocker glibenclamide inhibited an outward current in control neurons in a manner dependent on the pipette ATP concentration. Analysis of reversal potentials showed that this current is carried by K + ions. Outward current in cells from rats with peripheral nerve injury was not sensitive to modulators of KATP channels. Gabapentin, a putative KATP channel opener, had minimal effect on currents in either group of neurons. We conclude that normal primary afferent neurons express KATP channels that conduct current which is eliminated by peripheral nerve injury. Gabapentin does not affect this current significantly.

AB - ATP-sensitive potassium (KATP) currents were examined in dorsal root ganglion neurons from neuropathic and control rats using whole-cell voltage clamp recordings. KATP channel openers (diazoxide and pinacidil) enhanced, and the blocker glibenclamide inhibited an outward current in control neurons in a manner dependent on the pipette ATP concentration. Analysis of reversal potentials showed that this current is carried by K + ions. Outward current in cells from rats with peripheral nerve injury was not sensitive to modulators of KATP channels. Gabapentin, a putative KATP channel opener, had minimal effect on currents in either group of neurons. We conclude that normal primary afferent neurons express KATP channels that conduct current which is eliminated by peripheral nerve injury. Gabapentin does not affect this current significantly.

KW - ATP-sensitive potassium channels

KW - Chronic constriction injury

KW - Diazoxide

KW - Gabapentin

KW - Glibenclamide

KW - Neuropathy

KW - Pinacidil

KW - Primary afferent neurons

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ER -

ATP-sensitive potassium channels in rat primary afferent neurons: The effect of neuropathic injury and gabapentin

Abstract

Keywords

ASJC Scopus subject areas

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