Abstract
We report in this study the topography of a single layer of Cyt f and of a mixture of MGDG/Cyt f as a function of surface pressure (Π) and concentration of spreading solvent (ethanol). We used atomic force microscopy (AFM) to image the Langmuir-Blodgett (LB) films transferred from the air/water interface onto freshly cleaved mica. We observed that pure Cyt f tends to form assemblies with an average width of 12 nm at low ethanol concentration (20%), and domains about 100 nm wide at higher concentration (80%). However, in the mixed LB films (MGDG/Cyt f), the protein appear in the monomer form with a lateral dimension of approx. 3 nm. From these results, we consider that Cyt f assemblies are formed by an octamer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 233-238 |
| Number of pages | 6 |
| Journal | Thin Solid Films |
| Volume | 353 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 29 1999 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Surfaces and Interfaces
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry
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Tazi, A., Boussaad, S., & Leblanc, R. M. (1999). Atomic force microscopy study of cytochrome f (Cyt f) and mixed monogalactosyldiacylglycerol (MGDG)/Cyt f Langmuir-Blodgett films. Thin Solid Films, 353(1), 233-238. https://doi.org/10.1016/S0040-6090(99)00398-3