Atomic force microscopy study of cytochrome f (Cyt f) and mixed monogalactosyldiacylglycerol (MGDG)/Cyt f Langmuir-Blodgett films

A. Tazi, S. Boussaad, Roger Leblanc

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report in this study the topography of a single layer of Cyt f and of a mixture of MGDG/Cyt f as a function of surface pressure (Π) and concentration of spreading solvent (ethanol). We used atomic force microscopy (AFM) to image the Langmuir-Blodgett (LB) films transferred from the air/water interface onto freshly cleaved mica. We observed that pure Cyt f tends to form assemblies with an average width of 12 nm at low ethanol concentration (20%), and domains about 100 nm wide at higher concentration (80%). However, in the mixed LB films (MGDG/Cyt f), the protein appear in the monomer form with a lateral dimension of approx. 3 nm. From these results, we consider that Cyt f assemblies are formed by an octamer.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalThin Solid Films
Volume353
Issue number1
DOIs
StatePublished - Sep 29 1999

Fingerprint

Cytochromes f
Langmuir Blodgett films
cytochromes
Langmuir-Blodgett films
Atomic force microscopy
atomic force microscopy
Proteins
assemblies
ethyl alcohol
Ethanol
mica
Topography
Mica
topography
monomers
Monomers
monogalactosyldiacylglycerol
proteins
Water
air

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Condensed Matter Physics
  • Surfaces and Interfaces

Cite this

Atomic force microscopy study of cytochrome f (Cyt f) and mixed monogalactosyldiacylglycerol (MGDG)/Cyt f Langmuir-Blodgett films. / Tazi, A.; Boussaad, S.; Leblanc, Roger.

In: Thin Solid Films, Vol. 353, No. 1, 29.09.1999, p. 233-238.

Research output: Contribution to journalArticle

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