Abstract
We report in this study the topography of a single layer of Cyt f and of a mixture of MGDG/Cyt f as a function of surface pressure (Π) and concentration of spreading solvent (ethanol). We used atomic force microscopy (AFM) to image the Langmuir-Blodgett (LB) films transferred from the air/water interface onto freshly cleaved mica. We observed that pure Cyt f tends to form assemblies with an average width of 12 nm at low ethanol concentration (20%), and domains about 100 nm wide at higher concentration (80%). However, in the mixed LB films (MGDG/Cyt f), the protein appear in the monomer form with a lateral dimension of approx. 3 nm. From these results, we consider that Cyt f assemblies are formed by an octamer.
| Original language | English |
|---|---|
| Pages (from-to) | 233-238 |
| Number of pages | 6 |
| Journal | Thin Solid Films |
| Volume | 353 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 29 1999 |
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ASJC Scopus subject areas
- Surfaces, Coatings and Films
- Condensed Matter Physics
- Surfaces and Interfaces
Cite this
Atomic force microscopy study of cytochrome f (Cyt f) and mixed monogalactosyldiacylglycerol (MGDG)/Cyt f Langmuir-Blodgett films. / Tazi, A.; Boussaad, S.; Leblanc, Roger.
In: Thin Solid Films, Vol. 353, No. 1, 29.09.1999, p. 233-238.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Atomic force microscopy study of cytochrome f (Cyt f) and mixed monogalactosyldiacylglycerol (MGDG)/Cyt f Langmuir-Blodgett films
AU - Tazi, A.
AU - Boussaad, S.
AU - Leblanc, Roger
PY - 1999/9/29
Y1 - 1999/9/29
N2 - We report in this study the topography of a single layer of Cyt f and of a mixture of MGDG/Cyt f as a function of surface pressure (Π) and concentration of spreading solvent (ethanol). We used atomic force microscopy (AFM) to image the Langmuir-Blodgett (LB) films transferred from the air/water interface onto freshly cleaved mica. We observed that pure Cyt f tends to form assemblies with an average width of 12 nm at low ethanol concentration (20%), and domains about 100 nm wide at higher concentration (80%). However, in the mixed LB films (MGDG/Cyt f), the protein appear in the monomer form with a lateral dimension of approx. 3 nm. From these results, we consider that Cyt f assemblies are formed by an octamer.
AB - We report in this study the topography of a single layer of Cyt f and of a mixture of MGDG/Cyt f as a function of surface pressure (Π) and concentration of spreading solvent (ethanol). We used atomic force microscopy (AFM) to image the Langmuir-Blodgett (LB) films transferred from the air/water interface onto freshly cleaved mica. We observed that pure Cyt f tends to form assemblies with an average width of 12 nm at low ethanol concentration (20%), and domains about 100 nm wide at higher concentration (80%). However, in the mixed LB films (MGDG/Cyt f), the protein appear in the monomer form with a lateral dimension of approx. 3 nm. From these results, we consider that Cyt f assemblies are formed by an octamer.
UR - http://www.scopus.com/inward/record.url?scp=0033331891&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033331891&partnerID=8YFLogxK
U2 - 10.1016/S0040-6090(99)00398-3
DO - 10.1016/S0040-6090(99)00398-3
M3 - Article
AN - SCOPUS:0033331891
VL - 353
SP - 233
EP - 238
JO - Thin Solid Films
JF - Thin Solid Films
SN - 0040-6090
IS - 1
ER -