Atomic force microscopy study of cytochrome f (Cyt f) and mixed monogalactosyldiacylglycerol (MGDG)/Cyt f Langmuir-Blodgett films

A. Tazi, S. Boussaad, R. M. Leblanc

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We report in this study the topography of a single layer of Cyt f and of a mixture of MGDG/Cyt f as a function of surface pressure (Π) and concentration of spreading solvent (ethanol). We used atomic force microscopy (AFM) to image the Langmuir-Blodgett (LB) films transferred from the air/water interface onto freshly cleaved mica. We observed that pure Cyt f tends to form assemblies with an average width of 12 nm at low ethanol concentration (20%), and domains about 100 nm wide at higher concentration (80%). However, in the mixed LB films (MGDG/Cyt f), the protein appear in the monomer form with a lateral dimension of approx. 3 nm. From these results, we consider that Cyt f assemblies are formed by an octamer.

Original languageEnglish (US)
Pages (from-to)233-238
Number of pages6
JournalThin Solid Films
Volume353
Issue number1
DOIs
StatePublished - Sep 29 1999

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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