Atomic force microscopy measurement of leukocyte-endothelial interaction

Xiaohui Zhang, Aileen Chen, Dina De Leon, Hong Li, Eisei Noiri, Vincent T. Moy, Michael S. Goligorsky

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Leukocyte adhesion to vascular endothelium is a key initiating step in the pathogenesis of many inflammatory diseases. In this study, we present real-time force measurements of the interaction between monocytic human promyelocytic leukemia cells (HL-60) cells and a monolayer of human umbilical vein endothelial cells (HUVECs) by using atomic force microscopy (AFM). The detachment of HL-60-HUVEC conjugates involved a series of rupture events with force transitions of 40-100 pN. The integrated force of these rupture events provided a quantitative measure of the adhesion strength on a whole cell level. The AFM measurements revealed that HL-60 adhesion is heightened in the borders formed by adjacent HUVECs. The average force and mechanical work required to detach a single HL-60 from the borders of a tumor necrosis factor-α -activated HUVEC layer were twice as high as those of the HUVEC bodies. HL-60 adhesion to the monolayer was significantly reduced by a monoclonal antibody against β1-integrins and partially inhibited by antibodies against selectins ICAM-1 and VCAM-1 but was not affected by anti-α vβ3. Interestingly, adhesion was also inhibited in a dose-dependent manner (IC50 ≈ 100 nM) by a cyclic arginine-glycine-aspartic acid (cRGD) peptide. This effect was mediated via interfering with the VLA-4-VCAM-1 binding. In parallel measurements, transmigration of HL-60 cells across a confluent HUVEC monolayer was inhibited by the cRGD peptide and by both anti-β1 and anti-α vβ3 antibodies. In conclusion, these data demonstrate the role played by β1-integrins in leukocyte-endothelial adhesion and transmigration and the role played by αvβ3 in transmigration, thus underscoring the high efficacy of cRGD peptide in blocking both the adhesion and transmigration of monocytes.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume286
Issue number1 55-1
StatePublished - Jan 1 2004

Fingerprint

Atomic Force Microscopy
Human Umbilical Vein Endothelial Cells
Leukocytes
Vascular Cell Adhesion Molecule-1
HL-60 Cells
Integrins
Rupture
Integrin alpha4beta1
Transendothelial and Transepithelial Migration
Selectins
Vascular Endothelium
Intercellular Adhesion Molecule-1
Inhibitory Concentration 50
Monocytes
Anti-Idiotypic Antibodies
Leukemia
Tumor Necrosis Factor-alpha
Monoclonal Antibodies
cyclic arginine-glycine-aspartic acid peptide
Antibodies

Keywords

  • Arginine-glycine-aspartic acid peptide
  • Cell-cell adhesion
  • Integrins

ASJC Scopus subject areas

  • Physiology

Cite this

Atomic force microscopy measurement of leukocyte-endothelial interaction. / Zhang, Xiaohui; Chen, Aileen; De Leon, Dina; Li, Hong; Noiri, Eisei; Moy, Vincent T.; Goligorsky, Michael S.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 286, No. 1 55-1, 01.01.2004.

Research output: Contribution to journalArticle

Zhang, Xiaohui ; Chen, Aileen ; De Leon, Dina ; Li, Hong ; Noiri, Eisei ; Moy, Vincent T. ; Goligorsky, Michael S. / Atomic force microscopy measurement of leukocyte-endothelial interaction. In: American Journal of Physiology - Heart and Circulatory Physiology. 2004 ; Vol. 286, No. 1 55-1.
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