ATAD3 controls mitochondrial cristae structure in mouse muscle, influencing mtDNA replication and cholesterol levels

Susana Peralta, Steffi Goffart, Sion L. Williams, Francisca Diaz, Sofia Garcia, Nadee Nissanka, Estela Area-Gomez, Jaakko Pohjoismäki, Carlos T. Moraes

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Mutations in the mitochondrial inner membrane ATPase ATAD3A result in neurological syndromes in humans. In mice, the ubiquitous disruption of Atad3 (also known as Atad3a) was embryonic lethal, but a skeletal muscle-specific conditional knockout (KO) was viable. At birth, ATAD3 muscle KO mice had normal weight, but from 2 months onwards they showed progressive motor-impaired coordination and weakness. Loss of ATAD3 caused early and severe mitochondrial structural abnormalities, mitochondrial proliferation and muscle atrophy. There was dramatic reduction in mitochondrial cristae junctions and overall cristae morphology. The lack of mitochondrial cristae was accompanied by a reduction in high molecular weight mitochondrial contact site and cristae organizing system (MICOS) complexes, and to a lesser extent in OPA1. Moreover, muscles lacking ATAD3 showed altered cholesterol metabolism, accumulation of mitochondrial DNA (mtDNA) replication intermediates, progressive mtDNA depletion and deletions. Unexpectedly, decreases in the levels of some OXPHOS components occurred after cristae destabilization, indicating that ATAD3 is not crucial for mitochondrial translation, as previously suggested. Our results show a critical early role of ATAD3 in regulating mitochondrial inner membrane structure, leading to secondary defects in mtDNA replication and complex V and cholesterol levels in postmitotic tissue.

Original languageEnglish (US)
Article numberjcs217075
JournalJournal of Cell Science
Volume131
Issue number13
DOIs
StatePublished - Jul 1 2018

Keywords

  • ATAD3
  • Cholesterol
  • Cristae
  • MICOS complex
  • Mitochondria
  • Muscle

ASJC Scopus subject areas

  • Cell Biology

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