Asymmetrical localization of mRNAs in enterocytes of human jejunum

Jay A. Barth, Wei Li, Stephen D. Krasinski, Robert K. Montgomery, Menno Verhave, Richard J. Grand

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Intracellular localization of specific mRNAs is known to be a mechanism for targeting proteins to specific sites within the cell. Previous studies from this laboratory have demonstrated co-localization of mRNAs and proteins for a number of genes in absorptive enterocytes of fetal rat intestine. The present study was undertaken to examine in human enterocytes the intracellular localization patterns of mRNAs for the microvillous membrane proteins lactase-phlorizin hydrolase (LPH), sucrase-isomaltase (SI), and intestinal alkaline phosphatase (IAP), and the cytoskeletal protein β- actin. In sections of human jejunum. mRNAs were localized by in situ hybridization using digoxigenin-labeled antisense RNA probes. Both LPH and SI mRNAs were localized to the apical region of villous enterocytes, whereas IAP and β-actin mRNAs were detected both apically and basally relative to the nucleus. Therefore, in contrast to LPH, SI, and β-actin mRNAs, which co- localize with their epcoded proteins, that of IAP is present in the basal region of the cell where IAP protein has not directly been demonstrated to be present. Absorptive enterocytes from humans possess the mechanisms for intracellutar mRNA localization, but not all mRNAs co-localize with their encoded proteins.

Original languageEnglish (US)
Pages (from-to)335-343
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume46
Issue number3
StatePublished - Mar 1998
Externally publishedYes

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Keywords

  • β actin
  • Enterocytes
  • Intestinal alkatine phosphatase
  • Lactase-phlorizin hydrolase
  • mRNA localization
  • Sucrase- isomaltase

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Barth, J. A., Li, W., Krasinski, S. D., Montgomery, R. K., Verhave, M., & Grand, R. J. (1998). Asymmetrical localization of mRNAs in enterocytes of human jejunum. Journal of Histochemistry and Cytochemistry, 46(3), 335-343.