Asymmetrical displacement currents in the membrane of frog myelinated nerve: Early time course and effects of membrane potential

W. Nonner, E. Rojas, R. Stämpfli

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

1. Asymmetrical displacement currents were studied in myelinated nerve fibres from Rana esculenta with a voltage clamp technique. 2. For brief pulses symmetrical with respect to a holding potential of -97 mV, the asymmetry current flowing during pulses (on-response) exhibited a rising phase to a peak followed by an approximately exponential decline. After the pulses the rising phase in the off-response could not be resolved; the time constant varied about 2-fold with either size or duration of the pulse. 3. For longer pulses a second slower component could be detected both in on- and off-responses. 4. The rapidly declining on- and off-responses associated with brief pulses carried about the same charges Qon and Qoff. Increasing the duration of the pulse reduced Qoff. For all pulses tested Qoff approached about one fifth of Qmax. The reduction of Qoff was roughly characterised by time constants ranging between 1.5 and 0.5 ms for potentials between -25 and +23 mV. Analysis of individual membrane currents confirmed that the capacity current after depolarizing pulses decreased with pulse length. 5. The effects of membrane potential on asymmetry current were studied by varying the level from which pulses were applied during 46.9 ms prepulses in the range from -97 to -29 mV. The fast and slow components of asymmetry current were affected differently. For potentials more positive than -90 mV the fast on-response was reduced and reversed its sign at a potential 25 mV more negative than the potential estimated from the steady-state charge distribution measured from -97 mV.

Original languageEnglish
Pages (from-to)75-85
Number of pages11
JournalPflügers Archiv European Journal of Physiology
Volume375
Issue number1
DOIs
StatePublished - Jun 1 1978
Externally publishedYes

Fingerprint

Anura
Membrane Potentials
Rana esculenta
Membranes
Myelinated Nerve Fibers
Charge distribution
Clamping devices
Patch-Clamp Techniques
Fibers
Electric potential

Keywords

  • Gating current
  • Myelinated nerve
  • Voltage clamp

ASJC Scopus subject areas

  • Physiology

Cite this

Asymmetrical displacement currents in the membrane of frog myelinated nerve : Early time course and effects of membrane potential. / Nonner, W.; Rojas, E.; Stämpfli, R.

In: Pflügers Archiv European Journal of Physiology, Vol. 375, No. 1, 01.06.1978, p. 75-85.

Research output: Contribution to journalArticle

@article{e3a18ab9690c4a1a8e0ca3a566596cbc,
title = "Asymmetrical displacement currents in the membrane of frog myelinated nerve: Early time course and effects of membrane potential",
abstract = "1. Asymmetrical displacement currents were studied in myelinated nerve fibres from Rana esculenta with a voltage clamp technique. 2. For brief pulses symmetrical with respect to a holding potential of -97 mV, the asymmetry current flowing during pulses (on-response) exhibited a rising phase to a peak followed by an approximately exponential decline. After the pulses the rising phase in the off-response could not be resolved; the time constant varied about 2-fold with either size or duration of the pulse. 3. For longer pulses a second slower component could be detected both in on- and off-responses. 4. The rapidly declining on- and off-responses associated with brief pulses carried about the same charges Qon and Qoff. Increasing the duration of the pulse reduced Qoff. For all pulses tested Qoff approached about one fifth of Qmax. The reduction of Qoff was roughly characterised by time constants ranging between 1.5 and 0.5 ms for potentials between -25 and +23 mV. Analysis of individual membrane currents confirmed that the capacity current after depolarizing pulses decreased with pulse length. 5. The effects of membrane potential on asymmetry current were studied by varying the level from which pulses were applied during 46.9 ms prepulses in the range from -97 to -29 mV. The fast and slow components of asymmetry current were affected differently. For potentials more positive than -90 mV the fast on-response was reduced and reversed its sign at a potential 25 mV more negative than the potential estimated from the steady-state charge distribution measured from -97 mV.",
keywords = "Gating current, Myelinated nerve, Voltage clamp",
author = "W. Nonner and E. Rojas and R. St{\"a}mpfli",
year = "1978",
month = "6",
day = "1",
doi = "10.1007/BF00584151",
language = "English",
volume = "375",
pages = "75--85",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Asymmetrical displacement currents in the membrane of frog myelinated nerve

T2 - Early time course and effects of membrane potential

AU - Nonner, W.

AU - Rojas, E.

AU - Stämpfli, R.

PY - 1978/6/1

Y1 - 1978/6/1

N2 - 1. Asymmetrical displacement currents were studied in myelinated nerve fibres from Rana esculenta with a voltage clamp technique. 2. For brief pulses symmetrical with respect to a holding potential of -97 mV, the asymmetry current flowing during pulses (on-response) exhibited a rising phase to a peak followed by an approximately exponential decline. After the pulses the rising phase in the off-response could not be resolved; the time constant varied about 2-fold with either size or duration of the pulse. 3. For longer pulses a second slower component could be detected both in on- and off-responses. 4. The rapidly declining on- and off-responses associated with brief pulses carried about the same charges Qon and Qoff. Increasing the duration of the pulse reduced Qoff. For all pulses tested Qoff approached about one fifth of Qmax. The reduction of Qoff was roughly characterised by time constants ranging between 1.5 and 0.5 ms for potentials between -25 and +23 mV. Analysis of individual membrane currents confirmed that the capacity current after depolarizing pulses decreased with pulse length. 5. The effects of membrane potential on asymmetry current were studied by varying the level from which pulses were applied during 46.9 ms prepulses in the range from -97 to -29 mV. The fast and slow components of asymmetry current were affected differently. For potentials more positive than -90 mV the fast on-response was reduced and reversed its sign at a potential 25 mV more negative than the potential estimated from the steady-state charge distribution measured from -97 mV.

AB - 1. Asymmetrical displacement currents were studied in myelinated nerve fibres from Rana esculenta with a voltage clamp technique. 2. For brief pulses symmetrical with respect to a holding potential of -97 mV, the asymmetry current flowing during pulses (on-response) exhibited a rising phase to a peak followed by an approximately exponential decline. After the pulses the rising phase in the off-response could not be resolved; the time constant varied about 2-fold with either size or duration of the pulse. 3. For longer pulses a second slower component could be detected both in on- and off-responses. 4. The rapidly declining on- and off-responses associated with brief pulses carried about the same charges Qon and Qoff. Increasing the duration of the pulse reduced Qoff. For all pulses tested Qoff approached about one fifth of Qmax. The reduction of Qoff was roughly characterised by time constants ranging between 1.5 and 0.5 ms for potentials between -25 and +23 mV. Analysis of individual membrane currents confirmed that the capacity current after depolarizing pulses decreased with pulse length. 5. The effects of membrane potential on asymmetry current were studied by varying the level from which pulses were applied during 46.9 ms prepulses in the range from -97 to -29 mV. The fast and slow components of asymmetry current were affected differently. For potentials more positive than -90 mV the fast on-response was reduced and reversed its sign at a potential 25 mV more negative than the potential estimated from the steady-state charge distribution measured from -97 mV.

KW - Gating current

KW - Myelinated nerve

KW - Voltage clamp

UR - http://www.scopus.com/inward/record.url?scp=0017855008&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017855008&partnerID=8YFLogxK

U2 - 10.1007/BF00584151

DO - 10.1007/BF00584151

M3 - Article

C2 - 308219

AN - SCOPUS:0017855008

VL - 375

SP - 75

EP - 85

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 1

ER -