A31P-Nuclear magnetic resonance study of intermittent warm blood cardioplegia

Ganghong Tian, Bo Xiang, Keith W. Butler, Antonio M. Calafiore, Andrea Mezzetti, Tomas Salerno, Roxanne Deslauriers

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This study was designed to assess the effects of intermittent warm blood cardioplegia on myocardial energy metabolites, intracellular pH, and contractile function. The isolated blood-perfused pig hearts were divided into three groups. After 30 minutes of control perfusion, the hearts in group 1 ( n = 10) received 90 minutes of continuous warm (37° C) blood cardioplegia; the hearts in group 2 ( n = 9) received six 5-minute periods of warm blood cardioplegia, interrupted by six 10-minute episodes of ischemia (37° C). The hearts were then reperfused for 30 minutes. The hearts in group 3 underwent 150 minutes of control perfusion without cardioplegia or ischemic episodes. Phosphorus 31-nuclear magnetic resonance spectra showed that a 10-minute interruption of warm blood cardioplegia decreased phosphocreatine levels and intracellular pH by approximately 47% ( p < 0.01) and 0.12 unit ( p < 0.05), respectively, and increased inorganic phosphate levels by approximately 87%, whereas resumption of cardioplegia for 5 minutes resulted in almost 100% recovery of phosphocreatine and inorganic phosphate levels and intracellular pH. More important, subsequent interruptions did not result in any cumulative changes in phosphocreatine level, inorganic phosphate level, or intracellular pH beyond those changes observed after the initial cardioplegic interruption. Moreover, during reperfusion there were no significant differences in adenosine triphosphate and phosphocreatine levels among the three groups of hearts. Furthermore, hearts from groups 1 and 2 showed comparable recovery of contractile function. These results indicate that six 10-minute interruptions and six 5-minute restorations of warm blood cardioplegia caused only mild and reversible changes in myocardial energy metabolites and intracellular pH and these changes were not cumulative. This study suggests that antegrade intermittent warm blood cardioplegia may provide as much myocardial protection as does antegrade continuous warm blood cardioplegia in the normal heart . (J THORAC CARDIOVASC SURG 1995;109:1155-63).

Original languageEnglish
Pages (from-to)1155-1163
Number of pages9
JournalThe Journal of thoracic and cardiovascular surgery
Volume109
Issue number6
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Induced Heart Arrest
Magnetic Resonance Spectroscopy
Phosphocreatine
Phosphates
Perfusion
Recovery of Function
Phosphorus
Reperfusion
Swine
Ischemia
Adenosine Triphosphate

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

A31P-Nuclear magnetic resonance study of intermittent warm blood cardioplegia. / Tian, Ganghong; Xiang, Bo; Butler, Keith W.; Calafiore, Antonio M.; Mezzetti, Andrea; Salerno, Tomas; Deslauriers, Roxanne.

In: The Journal of thoracic and cardiovascular surgery, Vol. 109, No. 6, 01.01.1995, p. 1155-1163.

Research output: Contribution to journalArticle

Tian, Ganghong ; Xiang, Bo ; Butler, Keith W. ; Calafiore, Antonio M. ; Mezzetti, Andrea ; Salerno, Tomas ; Deslauriers, Roxanne. / A31P-Nuclear magnetic resonance study of intermittent warm blood cardioplegia. In: The Journal of thoracic and cardiovascular surgery. 1995 ; Vol. 109, No. 6. pp. 1155-1163.
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