Astrocyte swelling in liver failure

role of glutamine and benzodiazepines.

Michael D Norenberg, A. S. Bender

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

We examined the effect of methionine sulfoximine (MSO) and peripheral benzodiazepine (BZD) ligands on ammonia-induced swelling of primary astrocyte cultures. Swelling was completely abolished by co-treatment with MSO, an inhibitor of glutamine synthetase. We also established that many of the effects caused by ammonia, including the reduction in K+ uptake, increase in Cl- uptake and reduction in myo-inositol uptake were diminished by co-treatment with MSO. Agonists of the peripheral-type BZD receptor aggravated ammonia-induced swelling, whereas a peripheral BZD receptor blocker, PK 11195, diminished the extent of swelling. Our findings implicate glutamine and the peripheral-type benzodiazepine receptor in the pathogenesis of the edema associated with fulminant hepatic failure.

Original languageEnglish
Pages (from-to)24-27
Number of pages4
JournalActa Neurochirurgica, Supplement
Volume60
StatePublished - Jan 1 1994

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Methionine Sulfoximine
glutamine
Liver Failure
GABA-A Receptors
Glutamine
Benzodiazepines
Ammonia
liver
Astrocytes
swelling
methionine
Liver
Swelling
ammonia
Glutamate-Ammonia Ligase
Acute Liver Failure
Inositol
inositols
edema
pathogenesis

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Neurology

Cite this

Astrocyte swelling in liver failure : role of glutamine and benzodiazepines. / Norenberg, Michael D; Bender, A. S.

In: Acta Neurochirurgica, Supplement, Vol. 60, 01.01.1994, p. 24-27.

Research output: Contribution to journalArticle

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