Association of the viral oncoprotein STP-C488 with cellular ras

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93 Scopus citations

Abstract

The STP-C488 oncogene of herpesvirus saimiri has transforming activity independent of the rest of the viral genome. We now demonstrate that STP- C488 associates with cellular ras in transformed cells. Mutations that disrupted this association with ras disrupted the transforming ability of the STP-C488 oncogene. Binding assays showed that STP-C488 was capable of competing with raf-1 for binding to ras. Expression of STP-C488 activated the ras signaling pathway as evidenced by a two- to fourfold increase in the ratio of ras-GTP to ras-GDP and by the constitutive activation of mitogen- activated protein kinase. Consistent with an activation of signaling through ras, STP-C488 expression induced ras-dependent neurite outgrowth in PC12 cells. STP-C488 is the first virus-encoded protein shown to achieve oncogenic transformation via association with cellular ras.

Original languageEnglish (US)
Pages (from-to)6506-6512
Number of pages7
JournalMolecular and cellular biology
Volume15
Issue number12
DOIs
StatePublished - Dec 1995

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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