Abstract
The STP-C488 oncogene of herpesvirus saimiri has transforming activity independent of the rest of the viral genome. We now demonstrate that STP- C488 associates with cellular ras in transformed cells. Mutations that disrupted this association with ras disrupted the transforming ability of the STP-C488 oncogene. Binding assays showed that STP-C488 was capable of competing with raf-1 for binding to ras. Expression of STP-C488 activated the ras signaling pathway as evidenced by a two- to fourfold increase in the ratio of ras-GTP to ras-GDP and by the constitutive activation of mitogen- activated protein kinase. Consistent with an activation of signaling through ras, STP-C488 expression induced ras-dependent neurite outgrowth in PC12 cells. STP-C488 is the first virus-encoded protein shown to achieve oncogenic transformation via association with cellular ras.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6506-6512 |
| Number of pages | 7 |
| Journal | Molecular and Cellular Biology |
| Volume | 15 |
| Issue number | 12 |
| State | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Genetics
- Molecular Biology
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Association of the viral oncoprotein STP-C488 with cellular ras. / Jung, J. U.; Desrosiers, Ronald Charles.
In: Molecular and Cellular Biology, Vol. 15, No. 12, 1995, p. 6506-6512.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of the viral oncoprotein STP-C488 with cellular ras
AU - Jung, J. U.
AU - Desrosiers, Ronald Charles
PY - 1995
Y1 - 1995
N2 - The STP-C488 oncogene of herpesvirus saimiri has transforming activity independent of the rest of the viral genome. We now demonstrate that STP- C488 associates with cellular ras in transformed cells. Mutations that disrupted this association with ras disrupted the transforming ability of the STP-C488 oncogene. Binding assays showed that STP-C488 was capable of competing with raf-1 for binding to ras. Expression of STP-C488 activated the ras signaling pathway as evidenced by a two- to fourfold increase in the ratio of ras-GTP to ras-GDP and by the constitutive activation of mitogen- activated protein kinase. Consistent with an activation of signaling through ras, STP-C488 expression induced ras-dependent neurite outgrowth in PC12 cells. STP-C488 is the first virus-encoded protein shown to achieve oncogenic transformation via association with cellular ras.
AB - The STP-C488 oncogene of herpesvirus saimiri has transforming activity independent of the rest of the viral genome. We now demonstrate that STP- C488 associates with cellular ras in transformed cells. Mutations that disrupted this association with ras disrupted the transforming ability of the STP-C488 oncogene. Binding assays showed that STP-C488 was capable of competing with raf-1 for binding to ras. Expression of STP-C488 activated the ras signaling pathway as evidenced by a two- to fourfold increase in the ratio of ras-GTP to ras-GDP and by the constitutive activation of mitogen- activated protein kinase. Consistent with an activation of signaling through ras, STP-C488 expression induced ras-dependent neurite outgrowth in PC12 cells. STP-C488 is the first virus-encoded protein shown to achieve oncogenic transformation via association with cellular ras.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028788824&partnerID=8YFLogxK
M3 - Article
C2 - 8524215
AN - SCOPUS:0028788824
VL - 15
SP - 6506
EP - 6512
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 12
ER -