Association of reactive nitrogen species metabolites, myeloperoxidase, and airway inflammation in lung transplants

J. A. De Andrade, J. D. Christie, C. B. Alexander, K. R. Young, D. C. McGiffin, G. L. Zorn, Robert Jackson

Research output: Contribution to journalArticle

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Abstract

Background: We have previously reported that patients who had single or double lung transplants had higher concentrations than controls of nitrite and nitrate, which are metabolites of reactive nitrogen species (RNS), in bronchoalveolar lavage fluid (BALF) and serum. Methods: This study investigates implications of RNS metabolites as markers of airway inflammation in a distinct group of lung transplant patients (n=40). All patients underwent spirometry, routine surveillance transbronchial lung biopsies, and bronchoalveolar lavage as required by clinical protocol. Four normal controls also had bronchoscopy for measurement of BALF nitrite (NO2-) and nitrate (NO3-). BALF NO2- and NO3-, myeloperoxidase (MPO), protein, and urea were assayed. Total nitrite (NO2- plus enzymatically reduced NO3-) and urea were measured in serum. Results: BALF RNS metabolites were mainly NO3-. Forced expiratory volume in 1 s (FEV1) obtained near bronchoscopy was compared with best postoperative FEV1. Total nitrite in transplant patients' BALF and serum were 3.8±0.2 and 49±5 μM, respectively. Total nitrite in controls' BALF and serum were 2.2±0.7 and 19±2 μM, respectively (P≤0.05 compared with transplant values). Serum total nitrite correlated (Pearson product moment) with percentage of neutrophils in BALF (R=0.650, P≤0.0001), MPO (R=0.431, P=0.0055), change in FEV1 from baseline (ΔFEV1) (R=-0.348, P=0.0298), and days after transplantation (R=0.345, P=0.0294). None of the associated variables, airway inflammation (quantified as a score, "B"), ΔFEV1, serum, or BALF total nitrite, were explained by infection. Univariate analysis of airway inflammation in patients showed that it was associated with BALF neutrophils, ΔFEV1, and serum total nitrite. Conclusions: Serum nitrite appears to reflect the degree of airway inflammation in this lung-transplant study group.

Original languageEnglish
Pages (from-to)166-172
Number of pages7
JournalJournal of Investigative Medicine
Volume49
Issue number2
StatePublished - Mar 14 2001
Externally publishedYes

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Reactive Nitrogen Species
Transplants
Bronchoalveolar Lavage Fluid
Metabolites
Nitrites
Peroxidase
Pneumonia
Forced Expiratory Volume
Fluids
Serum
Bronchoscopy
Bronchoalveolar Lavage
Inflammation
Nitrates
Urea
Neutrophils
Lung
Biopsy
Spirometry
Clinical Protocols

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

De Andrade, J. A., Christie, J. D., Alexander, C. B., Young, K. R., McGiffin, D. C., Zorn, G. L., & Jackson, R. (2001). Association of reactive nitrogen species metabolites, myeloperoxidase, and airway inflammation in lung transplants. Journal of Investigative Medicine, 49(2), 166-172.

Association of reactive nitrogen species metabolites, myeloperoxidase, and airway inflammation in lung transplants. / De Andrade, J. A.; Christie, J. D.; Alexander, C. B.; Young, K. R.; McGiffin, D. C.; Zorn, G. L.; Jackson, Robert.

In: Journal of Investigative Medicine, Vol. 49, No. 2, 14.03.2001, p. 166-172.

Research output: Contribution to journalArticle

De Andrade, JA, Christie, JD, Alexander, CB, Young, KR, McGiffin, DC, Zorn, GL & Jackson, R 2001, 'Association of reactive nitrogen species metabolites, myeloperoxidase, and airway inflammation in lung transplants', Journal of Investigative Medicine, vol. 49, no. 2, pp. 166-172.
De Andrade JA, Christie JD, Alexander CB, Young KR, McGiffin DC, Zorn GL et al. Association of reactive nitrogen species metabolites, myeloperoxidase, and airway inflammation in lung transplants. Journal of Investigative Medicine. 2001 Mar 14;49(2):166-172.
De Andrade, J. A. ; Christie, J. D. ; Alexander, C. B. ; Young, K. R. ; McGiffin, D. C. ; Zorn, G. L. ; Jackson, Robert. / Association of reactive nitrogen species metabolites, myeloperoxidase, and airway inflammation in lung transplants. In: Journal of Investigative Medicine. 2001 ; Vol. 49, No. 2. pp. 166-172.
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abstract = "Background: We have previously reported that patients who had single or double lung transplants had higher concentrations than controls of nitrite and nitrate, which are metabolites of reactive nitrogen species (RNS), in bronchoalveolar lavage fluid (BALF) and serum. Methods: This study investigates implications of RNS metabolites as markers of airway inflammation in a distinct group of lung transplant patients (n=40). All patients underwent spirometry, routine surveillance transbronchial lung biopsies, and bronchoalveolar lavage as required by clinical protocol. Four normal controls also had bronchoscopy for measurement of BALF nitrite (NO2-) and nitrate (NO3-). BALF NO2- and NO3-, myeloperoxidase (MPO), protein, and urea were assayed. Total nitrite (NO2- plus enzymatically reduced NO3-) and urea were measured in serum. Results: BALF RNS metabolites were mainly NO3-. Forced expiratory volume in 1 s (FEV1) obtained near bronchoscopy was compared with best postoperative FEV1. Total nitrite in transplant patients' BALF and serum were 3.8±0.2 and 49±5 μM, respectively. Total nitrite in controls' BALF and serum were 2.2±0.7 and 19±2 μM, respectively (P≤0.05 compared with transplant values). Serum total nitrite correlated (Pearson product moment) with percentage of neutrophils in BALF (R=0.650, P≤0.0001), MPO (R=0.431, P=0.0055), change in FEV1 from baseline (ΔFEV1) (R=-0.348, P=0.0298), and days after transplantation (R=0.345, P=0.0294). None of the associated variables, airway inflammation (quantified as a score, {"}B{"}), ΔFEV1, serum, or BALF total nitrite, were explained by infection. Univariate analysis of airway inflammation in patients showed that it was associated with BALF neutrophils, ΔFEV1, and serum total nitrite. Conclusions: Serum nitrite appears to reflect the degree of airway inflammation in this lung-transplant study group.",
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AU - De Andrade, J. A.

AU - Christie, J. D.

AU - Alexander, C. B.

AU - Young, K. R.

AU - McGiffin, D. C.

AU - Zorn, G. L.

AU - Jackson, Robert

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N2 - Background: We have previously reported that patients who had single or double lung transplants had higher concentrations than controls of nitrite and nitrate, which are metabolites of reactive nitrogen species (RNS), in bronchoalveolar lavage fluid (BALF) and serum. Methods: This study investigates implications of RNS metabolites as markers of airway inflammation in a distinct group of lung transplant patients (n=40). All patients underwent spirometry, routine surveillance transbronchial lung biopsies, and bronchoalveolar lavage as required by clinical protocol. Four normal controls also had bronchoscopy for measurement of BALF nitrite (NO2-) and nitrate (NO3-). BALF NO2- and NO3-, myeloperoxidase (MPO), protein, and urea were assayed. Total nitrite (NO2- plus enzymatically reduced NO3-) and urea were measured in serum. Results: BALF RNS metabolites were mainly NO3-. Forced expiratory volume in 1 s (FEV1) obtained near bronchoscopy was compared with best postoperative FEV1. Total nitrite in transplant patients' BALF and serum were 3.8±0.2 and 49±5 μM, respectively. Total nitrite in controls' BALF and serum were 2.2±0.7 and 19±2 μM, respectively (P≤0.05 compared with transplant values). Serum total nitrite correlated (Pearson product moment) with percentage of neutrophils in BALF (R=0.650, P≤0.0001), MPO (R=0.431, P=0.0055), change in FEV1 from baseline (ΔFEV1) (R=-0.348, P=0.0298), and days after transplantation (R=0.345, P=0.0294). None of the associated variables, airway inflammation (quantified as a score, "B"), ΔFEV1, serum, or BALF total nitrite, were explained by infection. Univariate analysis of airway inflammation in patients showed that it was associated with BALF neutrophils, ΔFEV1, and serum total nitrite. Conclusions: Serum nitrite appears to reflect the degree of airway inflammation in this lung-transplant study group.

AB - Background: We have previously reported that patients who had single or double lung transplants had higher concentrations than controls of nitrite and nitrate, which are metabolites of reactive nitrogen species (RNS), in bronchoalveolar lavage fluid (BALF) and serum. Methods: This study investigates implications of RNS metabolites as markers of airway inflammation in a distinct group of lung transplant patients (n=40). All patients underwent spirometry, routine surveillance transbronchial lung biopsies, and bronchoalveolar lavage as required by clinical protocol. Four normal controls also had bronchoscopy for measurement of BALF nitrite (NO2-) and nitrate (NO3-). BALF NO2- and NO3-, myeloperoxidase (MPO), protein, and urea were assayed. Total nitrite (NO2- plus enzymatically reduced NO3-) and urea were measured in serum. Results: BALF RNS metabolites were mainly NO3-. Forced expiratory volume in 1 s (FEV1) obtained near bronchoscopy was compared with best postoperative FEV1. Total nitrite in transplant patients' BALF and serum were 3.8±0.2 and 49±5 μM, respectively. Total nitrite in controls' BALF and serum were 2.2±0.7 and 19±2 μM, respectively (P≤0.05 compared with transplant values). Serum total nitrite correlated (Pearson product moment) with percentage of neutrophils in BALF (R=0.650, P≤0.0001), MPO (R=0.431, P=0.0055), change in FEV1 from baseline (ΔFEV1) (R=-0.348, P=0.0298), and days after transplantation (R=0.345, P=0.0294). None of the associated variables, airway inflammation (quantified as a score, "B"), ΔFEV1, serum, or BALF total nitrite, were explained by infection. Univariate analysis of airway inflammation in patients showed that it was associated with BALF neutrophils, ΔFEV1, and serum total nitrite. Conclusions: Serum nitrite appears to reflect the degree of airway inflammation in this lung-transplant study group.

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