Association of murine splenocyte CD3 complex to the cytoskeleton: Absence of modulation by exogenous fatty acids

Michael D. Peck, Zhiming Li, Wenche Jy, Arthur J. Chu, Lilly Y.W. Bourguignon

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The cytoplasmic regions of the CD3 complex are presumably involved in signal transduction following ligand-receptor binding. We investigated the effects of incubating either stearic or oleic acid on the association of murine lymphocyte CD3 complex with the cytoskeleton. Both cytochalasin D, an inhibitor of microfilament formation, and W7, an inhibitor of calmodulin, inhibited capping of CD3. The association of CD3 with the cytoskeleton was confirmed by confocal laser scanning microscopy studies, which showed co-localization of the cross-linked CD3 receptors and the membrane attachment proteins ankyrin and fodrin. Although exogenous oleic acid increased plasma membrane fluidity, neither expression nor capping of CD3 receptors was increased. Nonetheless, oleic acid did increase uptake of tritiated thymidine after binding of anti-CD3 antibodies. Lymphoproliferation was progressively inhibited by both cytochalasin D and W7, confirming the importance of intact cytoskeleton for cellular activation.

Original languageEnglish (US)
Pages (from-to)531-537
Number of pages7
JournalCell Biology International
Volume20
Issue number8
DOIs
StatePublished - Aug 1996

Keywords

  • Cytoplasmic regions
  • Exogenous fatty acids
  • Murine splenocyte CD3 complex
  • Signal transduction

ASJC Scopus subject areas

  • Cell Biology

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