Association of Ki-ras with amplified DNA sequences, detected in human ovarian carcinomas by a modified in-gel renaturation assay

M. Fukumoto, R. D. Estensen, L. Sha, G. J. Oakley, L. B. Twiggs, L. L. Adcock, L. F. Carson, I. B. Roninson

Research output: Contribution to journalArticle

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Abstract

A modified in-gel DNA renaturation technique, which detects DNA sequences amplified >7-fold in human DNA, was used to analyze gene amplification in surgical specimens of primary and metastatic ovarian carcinomas. Amplified DNA sequences were detected in two of eight tumors. Hybridization of these samples with different oncogene probes revealed that both tumors contained an amplified Ki-ras gene, which in one case was coamplified with c-myc. In one of the tumors, Ki-ras was found to be amplified in both the primary tumor and three different metastatic nodules. No mutations at codons 12 or 61 of Ki-ras were detected in these tumors. No additional cases of Ki-ras or c-myc amplification were detected by Southern hybridization in the tumors that were found to be amplification negative by modified in-gel renaturation assays. These results indicate that gene amplification in ovarian carcinomas is likely to involve the Ki-ras oncogene.

Original languageEnglish
Pages (from-to)1693-1697
Number of pages5
JournalCancer Research
Volume49
Issue number7
StatePublished - May 17 1989
Externally publishedYes

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Gels
Carcinoma
Neoplasms
ras Genes
Gene Amplification
DNA
Oncogenes
Codon
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fukumoto, M., Estensen, R. D., Sha, L., Oakley, G. J., Twiggs, L. B., Adcock, L. L., ... Roninson, I. B. (1989). Association of Ki-ras with amplified DNA sequences, detected in human ovarian carcinomas by a modified in-gel renaturation assay. Cancer Research, 49(7), 1693-1697.

Association of Ki-ras with amplified DNA sequences, detected in human ovarian carcinomas by a modified in-gel renaturation assay. / Fukumoto, M.; Estensen, R. D.; Sha, L.; Oakley, G. J.; Twiggs, L. B.; Adcock, L. L.; Carson, L. F.; Roninson, I. B.

In: Cancer Research, Vol. 49, No. 7, 17.05.1989, p. 1693-1697.

Research output: Contribution to journalArticle

Fukumoto, M, Estensen, RD, Sha, L, Oakley, GJ, Twiggs, LB, Adcock, LL, Carson, LF & Roninson, IB 1989, 'Association of Ki-ras with amplified DNA sequences, detected in human ovarian carcinomas by a modified in-gel renaturation assay', Cancer Research, vol. 49, no. 7, pp. 1693-1697.
Fukumoto M, Estensen RD, Sha L, Oakley GJ, Twiggs LB, Adcock LL et al. Association of Ki-ras with amplified DNA sequences, detected in human ovarian carcinomas by a modified in-gel renaturation assay. Cancer Research. 1989 May 17;49(7):1693-1697.
Fukumoto, M. ; Estensen, R. D. ; Sha, L. ; Oakley, G. J. ; Twiggs, L. B. ; Adcock, L. L. ; Carson, L. F. ; Roninson, I. B. / Association of Ki-ras with amplified DNA sequences, detected in human ovarian carcinomas by a modified in-gel renaturation assay. In: Cancer Research. 1989 ; Vol. 49, No. 7. pp. 1693-1697.
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