Abstract
Approximately 96% of the lymphocytes of a patient with lymphocytic leukemia were found to possess surface membrane complement receptors, showed T-lymphocyte antigen, and formed spontaneous rosettes with sheep erythrocytes (SRBC). These cells showed the morphology of small- to medium-size lymphocytes and lymphoblasts. A lymphocyte transformation study showed that these T lymphocytes with complement receptors gave no response to phytohemagglutinin-P or concanavalin A and a low response to pokeweed mitogen (PWM) stimulation. No production of anti-SRBC antibody was detected with these lymphocytes as determined by a plaque assay after the lymphocytes had been stimulated by PWM. Using this plaque assay, these lymphocytes were cocultured with normal lymphocytes to test for their influence on normal antibody production. It was shown that the T lymphocytes with complement receptors were capable of providing helper activity for B-lymphocyte differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 125-135 |
| Number of pages | 11 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 13 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1979 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
Association of helper activity for B-lymphocyte differentiation with lymphocytes having both SRBC and complement receptors in a patient with lymphoproliferative disease. / Chiao, J. W.; Pahwa, Savita G; Arlin, Z. A.; Good, R. A.
In: Clinical Immunology and Immunopathology, Vol. 13, No. 2, 01.01.1979, p. 125-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Association of helper activity for B-lymphocyte differentiation with lymphocytes having both SRBC and complement receptors in a patient with lymphoproliferative disease
AU - Chiao, J. W.
AU - Pahwa, Savita G
AU - Arlin, Z. A.
AU - Good, R. A.
PY - 1979/1/1
Y1 - 1979/1/1
N2 - Approximately 96% of the lymphocytes of a patient with lymphocytic leukemia were found to possess surface membrane complement receptors, showed T-lymphocyte antigen, and formed spontaneous rosettes with sheep erythrocytes (SRBC). These cells showed the morphology of small- to medium-size lymphocytes and lymphoblasts. A lymphocyte transformation study showed that these T lymphocytes with complement receptors gave no response to phytohemagglutinin-P or concanavalin A and a low response to pokeweed mitogen (PWM) stimulation. No production of anti-SRBC antibody was detected with these lymphocytes as determined by a plaque assay after the lymphocytes had been stimulated by PWM. Using this plaque assay, these lymphocytes were cocultured with normal lymphocytes to test for their influence on normal antibody production. It was shown that the T lymphocytes with complement receptors were capable of providing helper activity for B-lymphocyte differentiation.
AB - Approximately 96% of the lymphocytes of a patient with lymphocytic leukemia were found to possess surface membrane complement receptors, showed T-lymphocyte antigen, and formed spontaneous rosettes with sheep erythrocytes (SRBC). These cells showed the morphology of small- to medium-size lymphocytes and lymphoblasts. A lymphocyte transformation study showed that these T lymphocytes with complement receptors gave no response to phytohemagglutinin-P or concanavalin A and a low response to pokeweed mitogen (PWM) stimulation. No production of anti-SRBC antibody was detected with these lymphocytes as determined by a plaque assay after the lymphocytes had been stimulated by PWM. Using this plaque assay, these lymphocytes were cocultured with normal lymphocytes to test for their influence on normal antibody production. It was shown that the T lymphocytes with complement receptors were capable of providing helper activity for B-lymphocyte differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0018656737&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018656737&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(79)90056-4
DO - 10.1016/0090-1229(79)90056-4
M3 - Article
C2 - 313300
AN - SCOPUS:0018656737
VL - 13
SP - 125
EP - 135
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 2
ER -