Abstract
Changes in the Ca2+ and Sr2+ activation properties of functionally skinned slow-twitch soleus fibers were measured and compared with those of normal fast-twitch extensor digitorum longus (EDL) following cross-reinnervation of soleus with the nerve to EDL. Most of the fibers showed either complete transformation of activation properties (66%) or remained unchanged (34%). The change in sensitivity to divalent cations was correlated with changes in the proteins present in fibers pooled on the basis of their activation properties. The banding patterns of the 35,000- and 37,500-dalton proteins (tropomyosin and troponin T) in cross-reinnervated soleus were correspondingly transformed to those of normal EDL. Slow and fast myosin light chains were present in the pooled cross-reinnervated fibers. Fiber distributions based on activation properties were confirmed by histochemical features. For the first time it has been demonstrated that cross-reinnervation produced changes in the activation properties of soleus fibers and associated changes in the regulatory proteins measured.
| Original language | English |
|---|---|
| Pages (from-to) | 219-229 |
| Number of pages | 11 |
| Journal | Pflügers Archiv European Journal of Physiology |
| Volume | 384 |
| Issue number | 3 |
| DOIs | |
| State | Published - Apr 1 1980 |
| Externally published | Yes |
Fingerprint
Keywords
- Calcium
- Cross-reinnervation
- Fiber types
- Myosin light chains
- Skinned fibers
- Troponin
ASJC Scopus subject areas
- Physiology
Cite this
Associated changes in Ca2+ and Sr2+ activation properties and fiber proteins in cross-reinnervated rabbit soleus. / Secrist, D. J.; Kerrick, W. Glenn.
In: Pflügers Archiv European Journal of Physiology, Vol. 384, No. 3, 01.04.1980, p. 219-229.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Associated changes in Ca2+ and Sr2+ activation properties and fiber proteins in cross-reinnervated rabbit soleus
AU - Secrist, D. J.
AU - Kerrick, W. Glenn
PY - 1980/4/1
Y1 - 1980/4/1
N2 - Changes in the Ca2+ and Sr2+ activation properties of functionally skinned slow-twitch soleus fibers were measured and compared with those of normal fast-twitch extensor digitorum longus (EDL) following cross-reinnervation of soleus with the nerve to EDL. Most of the fibers showed either complete transformation of activation properties (66%) or remained unchanged (34%). The change in sensitivity to divalent cations was correlated with changes in the proteins present in fibers pooled on the basis of their activation properties. The banding patterns of the 35,000- and 37,500-dalton proteins (tropomyosin and troponin T) in cross-reinnervated soleus were correspondingly transformed to those of normal EDL. Slow and fast myosin light chains were present in the pooled cross-reinnervated fibers. Fiber distributions based on activation properties were confirmed by histochemical features. For the first time it has been demonstrated that cross-reinnervation produced changes in the activation properties of soleus fibers and associated changes in the regulatory proteins measured.
AB - Changes in the Ca2+ and Sr2+ activation properties of functionally skinned slow-twitch soleus fibers were measured and compared with those of normal fast-twitch extensor digitorum longus (EDL) following cross-reinnervation of soleus with the nerve to EDL. Most of the fibers showed either complete transformation of activation properties (66%) or remained unchanged (34%). The change in sensitivity to divalent cations was correlated with changes in the proteins present in fibers pooled on the basis of their activation properties. The banding patterns of the 35,000- and 37,500-dalton proteins (tropomyosin and troponin T) in cross-reinnervated soleus were correspondingly transformed to those of normal EDL. Slow and fast myosin light chains were present in the pooled cross-reinnervated fibers. Fiber distributions based on activation properties were confirmed by histochemical features. For the first time it has been demonstrated that cross-reinnervation produced changes in the activation properties of soleus fibers and associated changes in the regulatory proteins measured.
KW - Calcium
KW - Cross-reinnervation
KW - Fiber types
KW - Myosin light chains
KW - Skinned fibers
KW - Troponin
UR - http://www.scopus.com/inward/record.url?scp=0019001425&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019001425&partnerID=8YFLogxK
U2 - 10.1007/BF00584556
DO - 10.1007/BF00584556
M3 - Article
C2 - 7191084
AN - SCOPUS:0019001425
VL - 384
SP - 219
EP - 229
JO - Pflugers Archiv European Journal of Physiology
JF - Pflugers Archiv European Journal of Physiology
SN - 0031-6768
IS - 3
ER -