Assessments of injectable alginate particle-embedded fibrin hydrogels for soft tissue reconstruction

C. M. Hwang, B. Ay, D. L. Kaplan, J. P. Rubin, K. G. Marra, A. Atala, J. J. Yoo, S. J. Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Soft tissue reconstruction is often needed after massive traumatic damage or cancer removal. In this study, we developed a novel hybrid hydrogel system consisting of alginate particles and a fibrin matrix that could maintain tissue volume long term. Alginate particles were fabricated by mixing 5% alginate with a 20 mM calcium solution. Cells and these alginate particles were then embedded in fibrin (alginate-fibrin) hydrogels using a dual syringe mixer. Cell-hydrogel constructs were evaluated in terms of cell survival and proliferation in the constructs in vitro. The results indicated that cellular viability, spreading and proliferation in the alginate-fibrin hydrogels were significantly higher compared to constructs fabricated with fibrin or alginate only. In vivo explants showed that cells contained within fibrin-only hydrogels did not contribute to neo-tissue formation, and the fibrin was fully degraded within a 12 week period. In the alginate-fibrin system, higher cellularity and vascular ingrowth were observed in vivo. This resulted in neo-tissue formation in the alginate-fibrin hydrogels. These results demonstrate that fibrin may enhance cell proliferation and accelerate the formation of extracellular matrix proteins in the alginate-fibrin system, while the alginate particles could contribute to volume retention. This injectable hybrid system composed of degradable and non-degradable hydrogels may be a preferable approach to the repair of soft tissue defects.

Original languageEnglish
Article number014105
JournalBiomedical Materials (Bristol)
Volume8
Issue number1
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

Fingerprint

Hydrogels
Alginate
Fibrin
Particles (particulate matter)
Tissue
Injections
Hydrogel
Cell Proliferation
alginic acid
Syringes
Extracellular Matrix Proteins
Cell proliferation
Hybrid systems
Blood Vessels
Calcium
Cell Survival
Repair
Cells
Proteins
Defects

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biochemistry
  • Mechanics of Materials

Cite this

Hwang, C. M., Ay, B., Kaplan, D. L., Rubin, J. P., Marra, K. G., Atala, A., ... Lee, S. J. (2013). Assessments of injectable alginate particle-embedded fibrin hydrogels for soft tissue reconstruction. Biomedical Materials (Bristol), 8(1), [014105]. https://doi.org/10.1088/1748-6041/8/1/014105

Assessments of injectable alginate particle-embedded fibrin hydrogels for soft tissue reconstruction. / Hwang, C. M.; Ay, B.; Kaplan, D. L.; Rubin, J. P.; Marra, K. G.; Atala, A.; Yoo, J. J.; Lee, S. J.

In: Biomedical Materials (Bristol), Vol. 8, No. 1, 014105, 01.02.2013.

Research output: Contribution to journalArticle

Hwang, C. M. ; Ay, B. ; Kaplan, D. L. ; Rubin, J. P. ; Marra, K. G. ; Atala, A. ; Yoo, J. J. ; Lee, S. J. / Assessments of injectable alginate particle-embedded fibrin hydrogels for soft tissue reconstruction. In: Biomedical Materials (Bristol). 2013 ; Vol. 8, No. 1.
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